Simultaneous production of commercial enzymes using agro industrial residues by statistical approach.
Bacillus pumilus
banana peel
fruit clarifying agent
orange peel
solid state fermentation
Journal
Journal of the science of food and agriculture
ISSN: 1097-0010
Titre abrégé: J Sci Food Agric
Pays: England
ID NLM: 0376334
Informations de publication
Date de publication:
Apr 2019
Apr 2019
Historique:
received:
22
08
2018
revised:
16
10
2018
accepted:
17
10
2018
pubmed:
23
10
2018
medline:
19
4
2019
entrez:
23
10
2018
Statut:
ppublish
Résumé
Simultaneous production of commercial enzymes using agro-industrial residues by statistical approach is an important perspective in an industrial point of view. Despite the advantages of statistical methods optimization, the report on simultaneous production of pectinase and amylases are limited. The accumulation of agro-industrial residues causes serious environmental problems; however, citrus peel can be the important substrate for various enzymes production, including pectinase. These enzymes involving saccharification process and act as clarifying agent. In this study, orange peel and banana peel mixture were used as the suitable substrate for pectinase and amylase production using Bacillus pumilus in solid-state culture. The process parameters were optimized for simultaneous production of enzymes by a traditional-one-variable-at-a-time approach, a two level full factorial design, central composite design and response surface methodology. Among the selected variables, moisture content of the medium, pH and mineral supplement significantly influenced pectinase and amylase production. Pectinase production increased over 3-fold, whereas, 2-fold increase on amylase production was achieved after optimization by statistical approach. The purified pectinase exhibited maximal activity at pH 8.0, temperature of 60 °C and the molecular weight was 60 kDa. The purified amylase was highly active at pH 8.0, at 50 °C and the molecular weight was 37 kDa. The enzyme showed activity on fruit pulp in increasing clarity in orange and carrot juice and the saccharification of starch. Orange peel and banana peel mixture was effective as a solid medium for the simultaneous production of pectinase and amylase by Bacillus pumilus. Also, our statistical approach to optimize the medium components to yield more pectinase and amylase was fruitful and these enzymes showed appreciable results suitable for various applications. © 2018 Society of Chemical Industry.
Sections du résumé
BACKGROUND
BACKGROUND
Simultaneous production of commercial enzymes using agro-industrial residues by statistical approach is an important perspective in an industrial point of view. Despite the advantages of statistical methods optimization, the report on simultaneous production of pectinase and amylases are limited. The accumulation of agro-industrial residues causes serious environmental problems; however, citrus peel can be the important substrate for various enzymes production, including pectinase. These enzymes involving saccharification process and act as clarifying agent.
RESULTS
RESULTS
In this study, orange peel and banana peel mixture were used as the suitable substrate for pectinase and amylase production using Bacillus pumilus in solid-state culture. The process parameters were optimized for simultaneous production of enzymes by a traditional-one-variable-at-a-time approach, a two level full factorial design, central composite design and response surface methodology. Among the selected variables, moisture content of the medium, pH and mineral supplement significantly influenced pectinase and amylase production. Pectinase production increased over 3-fold, whereas, 2-fold increase on amylase production was achieved after optimization by statistical approach. The purified pectinase exhibited maximal activity at pH 8.0, temperature of 60 °C and the molecular weight was 60 kDa. The purified amylase was highly active at pH 8.0, at 50 °C and the molecular weight was 37 kDa. The enzyme showed activity on fruit pulp in increasing clarity in orange and carrot juice and the saccharification of starch.
CONCLUSION
CONCLUSIONS
Orange peel and banana peel mixture was effective as a solid medium for the simultaneous production of pectinase and amylase by Bacillus pumilus. Also, our statistical approach to optimize the medium components to yield more pectinase and amylase was fruitful and these enzymes showed appreciable results suitable for various applications. © 2018 Society of Chemical Industry.
Substances chimiques
Industrial Waste
0
Amylases
EC 3.2.1.-
Polygalacturonase
EC 3.2.1.15
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2685-2696Subventions
Organisme : King Saud University
Organisme : Deanship of Scientific Research
Informations de copyright
© 2018 Society of Chemical Industry.