Bioimprinting for multiplex luminescent detection of deoxynivalenol and zearalenone.

Bioimprinting Deoxynivalenol Immunoassay Quantum dots Silica coated quantum dot Zearalenone

Journal

Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R

Informations de publication

Date de publication:
15 Jan 2019
Historique:
received: 06 07 2018
revised: 08 09 2018
accepted: 11 09 2018
entrez: 24 10 2018
pubmed: 24 10 2018
medline: 24 10 2018
Statut: ppublish

Résumé

A sensitive tool for simultaneous quantitative determination of two analytes in a single spot with the use of a bioimprinted protein is presented for the first time. BSA is chosen as a scaffold for generation of binding sites specific towards two compounds. A multiplex immunosorbent assay for screening of two cereal-born mycotoxins, deoxynivalenol and zearalenone, in wheat and maize is realized with the use of fluorescent silica coated quantum dots as labels. Water-soluble fluorescent nanostructures consist of core/shell Cd-QDs enrobed in silica shells to ensure their solubility. The mycotoxins are simultaneously detected by scanning the assay outcome at two different wavelengths, since two QD@SiO

Identifiants

DOI: 10.1016/j.talanta.2018.09.042 PMID: 30348374
pubmed: 30348374
pii: S0039-9140(18)30950-0
doi: 10.1016/j.talanta.2018.09.042
pii:
doi:

Types de publication

Journal Article

Langues

eng

Pagination

169-174

Informations de copyright

Copyright © 2018 Elsevier B.V. All rights reserved.

Auteurs

Natalia Beloglazova (N)

Ghent University, Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ottergemsesteenweg 460, 9000 Ghent, Belgium; Saratov State University, Chemistry Faculty, Department of General and Inorganic Chemistry, Astrakhanskaya 83, 410012 Saratov, Russia. Electronic address: beloglazovanv@gmail.com.

Pieterjan Lenain (P)

Ghent University, Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ottergemsesteenweg 460, 9000 Ghent, Belgium.

Mickael Tessier (M)

Faculty of Sciences, Department of Inorganic and Physical Chemistry, Ghent University, Krijgslaan 281 S3, Ghent, Belgium; Center for Nano and Biophotonics, Ghent University, Krijgslaan 281 S3, 9000 Ghent, Belgium.

Irina Goryacheva (I)

Saratov State University, Chemistry Faculty, Department of General and Inorganic Chemistry, Astrakhanskaya 83, 410012 Saratov, Russia.

Zeger Hens (Z)

Faculty of Sciences, Department of Inorganic and Physical Chemistry, Ghent University, Krijgslaan 281 S3, Ghent, Belgium; Center for Nano and Biophotonics, Ghent University, Krijgslaan 281 S3, 9000 Ghent, Belgium.

Sarah De Saeger (S)

Ghent University, Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ottergemsesteenweg 460, 9000 Ghent, Belgium.

Classifications MeSH