Optimization and validation of a DHS-TD-GC-MS method to wineomics studies.
Experimental design
Gas chromatography-quadrupole mass spectrometry
Metabolomics
Method validation
Wine
Journal
Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R
Informations de publication
Date de publication:
15 Jan 2019
15 Jan 2019
Historique:
received:
19
06
2018
revised:
07
09
2018
accepted:
11
09
2018
entrez:
24
10
2018
pubmed:
24
10
2018
medline:
24
10
2018
Statut:
ppublish
Résumé
A dynamic headspace sorptive extraction (DHS) combined with thermal desorption (TD) and coupled with gas chromatography-mass spectrometry (GC/MS) was developed for the determination of forty-four volatile aroma metabolites (VAMs) which describe aroma fingerprints of wines. The response surface methodology (RSM) and a central composite design (CCD) was used to obtain the optimal values for the experimental extraction variables, and the results were assessed by an analysis of variance (ANOVA) and a principal component analysis (PCA). The VAMs exhibited optimal extraction with the high levels of salt concentration (1.5 g NaCl) and using an extraction temperature of 40 °C during 10 min, and a subsequent purge volume of 300 mL. Subsequently, the calibration curves were created for the quantification of each VAMs with seven levels of concentration obtaining a correlation coefficients (R
Identifiants
pubmed: 30348393
pii: S0039-9140(18)30946-9
doi: 10.1016/j.talanta.2018.09.032
pii:
doi:
Types de publication
Journal Article
Langues
eng
Pagination
301-307Informations de copyright
Copyright © 2018 Elsevier B.V. All rights reserved.