Characterization of Schinus lentiscifolius Marchand (Anacardiaceae) Bark Extract and Its Effects on Lymphocyte Oxidative Stress and Heat Shock Response.

Anacardiaceae / chemistry Animals Antioxidants / pharmacology Cell Survival / drug effects Chromatography, High Pressure Liquid / methods Chromatography, Thin Layer Female Flavonoids / analysis HSP70 Heat-Shock Proteins / metabolism Heat-Shock Response / drug effects Hydrogen Peroxide / metabolism Kaempferols / pharmacology Lipid Peroxidation / drug effects Lymphocytes / drug effects Oxidative Stress / drug effects Phenols / analysis Plant Bark / chemistry Plant Extracts / pharmacology Quercetin / pharmacology Rats, Wistar Spectrometry, Mass, Electrospray Ionization / methods Tandem Mass Spectrometry / methods Thiobarbituric Acid Reactive Substances / metabolism

HSP70 Schinus lentiscifolius antioxidant activity biological activity flavonoids lipid peroxidation oxidative stress

Journal

Chemistry & biodiversity

ISSN: 1612-1880

Titre abrégé: Chem Biodivers

Pays: Switzerland

ID NLM: 101197449

Informations de publication

Date de publication:
Jan 2019

Historique:

received: 26 06 2018

accepted: 23 10 2018

pubmed: 24 10 2018

medline: 12 3 2019

entrez: 24 10 2018

Statut: ppublish

Résumé

Schinus lentiscifolius Marchand has been used in folk medicine to treat immunoinflammatory related diseases, which are marked by OS and altered HSR. Our study aimed to evaluate OS and HSR in lymphocytes treated with S. lentiscifolius bark extracts. S. lentiscifolius barks were partitioned with solvents to obtain hexane (SL-HEX), ethyl acetate (SL-ACOET) and methanol (SL-MEOH) extracts, and the presence of bioactive compounds was evaluated by thin layer chromatography. Total phenols were measured by the Folin-Ciocalteu method and flavonoids were identified by HPLC-DAD-ESI-MS/MS. Antioxidant capacity was verified by DPPH method, cell viability by Trypan Blue method, lipid peroxidation by TBARS and HSP70 by immunoblotting. The SL-ACOET extract presented higher content of phenolic compounds and antioxidant activity in vitro. It was able to reduce lipid peroxidation levels in lymphocytes induced by H

Identifiants

DOI: 10.1002/cbdv.201800303 PMID: 30351529

pubmed: 30351529

doi: 10.1002/cbdv.201800303

doi:

Substances chimiques

Antioxidants 0

Flavonoids 0

HSP70 Heat-Shock Proteins 0

Kaempferols 0

Phenols 0

Plant Extracts 0

Thiobarbituric Acid Reactive Substances 0

kaempferol 731P2LE49E

Quercetin 9IKM0I5T1E

Hydrogen Peroxide BBX060AN9V

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

e1800303

Subventions

Organisme : Research Support Foundation of the State of Rio Grande do Sul

ID : ARD/PPP-2014 - FAPERGS

Organisme : Research Support Foundation of the State of Rio Grande do Sul

ID : #16/2551-00001196-6 to TGH