Comparative electrophysiological analysis of the bile acid-sensitive ion channel (BASIC) from different species suggests similar physiological functions.
Acid Sensing Ion Channels
/ metabolism
Amino Acids
/ metabolism
Animals
Bile Acids and Salts
/ metabolism
Calcium
/ metabolism
Cell Line
Electrophysiological Phenomena
/ physiology
HEK293 Cells
Humans
Mice
Oocytes
/ metabolism
Patch-Clamp Techniques
/ methods
Rats
Sodium
/ metabolism
Xenopus laevis
/ metabolism
ASIC
BASIC
Bile acid
Cation channel
ENaC
Journal
Pflugers Archiv : European journal of physiology
ISSN: 1432-2013
Titre abrégé: Pflugers Arch
Pays: Germany
ID NLM: 0154720
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
27
08
2018
accepted:
12
10
2018
revised:
11
10
2018
pubmed:
26
10
2018
medline:
21
3
2020
entrez:
25
10
2018
Statut:
ppublish
Résumé
Despite the identification of cholangiocytes in the liver and unipolar brush cells in the cerebellum as sites of expression, the physiological function of the bile acid-sensitive ion channel (BASIC) remains unknown. Rat BASIC (rBASIC) and mouse BASIC (mBASIC) share 97% of their amino acid sequence but show strikingly different biophysical properties. rBASIC is inactive at rest while mBASIC is constitutively active, when expressed in Xenopus oocytes. This conundrum rendered the identification of the physiological function even more difficult. In this study, we investigated the electrophysiological and pharmacological properties of BASIC from rat, mouse, and human in Hek293 cells using the patch clamp technique. Surprisingly, in Hek293 cells, rBASIC and mBASIC showed almost completely identical properties. Both are blocked by extracellular Ca
Identifiants
pubmed: 30353368
doi: 10.1007/s00424-018-2223-z
pii: 10.1007/s00424-018-2223-z
doi:
Substances chimiques
Acid Sensing Ion Channels
0
Amino Acids
0
Bile Acids and Salts
0
Sodium
9NEZ333N27
Calcium
SY7Q814VUP
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
329-336Références
J Biol Chem. 2010 Oct 1;285(40):30404-10
pubmed: 20656685
Mol Pharmacol. 2017 Dec;92(6):665-675
pubmed: 29025967
Nature. 2007 Sep 20;449(7160):316-23
pubmed: 17882215
Physiol Rep. 2017 Feb;5(3):
pubmed: 28193786
J Biol Chem. 2010 Apr 16;285(16):11958-65
pubmed: 20159980
BMC Biol. 2014 Oct 14;12:84
pubmed: 25312679
Pharmacol Rev. 2015;67(1):1-35
pubmed: 25287517
Nature. 1991 Feb 14;349(6310):588-93
pubmed: 1672038
Gastroenterology. 1997 Jan;112(1):226-35
pubmed: 8978363
Neuropharmacology. 2010 Jun;58(7):1045-53
pubmed: 20114056
J Biol Chem. 2016 Sep 16;291(38):19835-47
pubmed: 27489102
PLoS One. 2014 Oct 31;9(10):e111549
pubmed: 25360526
Nat Rev Neurosci. 2013 Jul;14(7):461-71
pubmed: 23783197
Channels (Austin). 2014;8(1):29-34
pubmed: 24365967
PLoS One. 2014 Mar 24;9(3):e91326
pubmed: 24663811
Channels (Austin). 2013 Jan 1;7(1):38-42
pubmed: 23064163
Mol Pharmacol. 2011 Nov;80(5):911-9
pubmed: 21828194
Nature. 1995 Dec 14;378(6558):730-3
pubmed: 7501021
FASEB J. 2012 Oct;26(10):4122-30
pubmed: 22735174
Nature. 2018 Mar 15;555(7696):397-401
pubmed: 29513651
J Physiol. 1999 Sep 1;519 Pt 2:323-33
pubmed: 10457052
FEBS Lett. 2000 Apr 14;471(2-3):205-10
pubmed: 10767424
Pflugers Arch. 2014 Feb;466(2):253-63
pubmed: 23842738
J Biol Chem. 2016 Nov 18;291(47):24551-24565
pubmed: 27679529
J Chromatogr. 1993 Nov 24;621(2):123-31
pubmed: 8294533
Nature. 1994 Feb 3;367(6462):463-7
pubmed: 8107805
Physiol Rev. 2002 Jul;82(3):735-67
pubmed: 12087134
Pflugers Arch. 2018 Jul;470(7):1087-1102
pubmed: 29589117
Mol Neurobiol. 2007 Dec;36(3):254-71
pubmed: 17955200
Can Med Assoc J. 1973 Aug 4;109(3):190-3
pubmed: 4728947