Nisin, a food preservative produced by Lactococcus lactis, affects the localization pattern of intermediate filament protein in HaCaT cells.
Bepridil
/ pharmacology
Calcium Channel Blockers
/ pharmacology
Cells, Cultured
Cytoskeleton
/ metabolism
Dose-Response Relationship, Drug
Food Preservatives
/ pharmacology
Humans
Intermediate Filament Proteins
/ metabolism
Keratin-17
/ metabolism
Keratin-5
/ metabolism
Keratinocytes
/ cytology
Lactococcus lactis
/ metabolism
Nisin
/ antagonists & inhibitors
Cytokeratin 17
Cytokeratin 5
HaCaT
Keratinocyte
Nisin
Journal
Anatomical science international
ISSN: 1447-073X
Titre abrégé: Anat Sci Int
Pays: Japan
ID NLM: 101154140
Informations de publication
Date de publication:
Mar 2019
Mar 2019
Historique:
received:
17
05
2017
accepted:
12
10
2018
pubmed:
26
10
2018
medline:
6
4
2019
entrez:
25
10
2018
Statut:
ppublish
Résumé
Nisin is a food preservative produced by Lactococcus lactis subsp. lactis. Previous blood biochemical research revealed that nisin has physiological effects in mammals; although the site of action has yet to be identified, keratinocytes have been proposed as a possible target. In this study, we investigated whether nisin affects keratinocytes by examining the effects on eukaryotic intermediate filaments in HaCaT human keratinocytes. Treatment with 93 μg/ml nisin for 24 h decreased the localization of the intermediate filament proteins cytokeratin (CK)5 and CK17 at the cell periphery, which were distributed in a limited area in a ring- or net-like shape. However, this was not observed upon treatment for 6 h. The results of a serial dilution assay revealed that the effect on CK17 localization depends on the nisin concentration and were observed at ≥47 μg/ml. Moreover, this effect was partially blocked by treatment with the calcium channel blocker bepridil. Thus, despite the long history of nisin as being safe for humans, it has measurable effects on the keratinocyte cytoskeleton. Our findings also indicate that CK5 and CK17 can serve as markers for evaluating the effects of nisin on keratinocytes.
Identifiants
pubmed: 30353456
doi: 10.1007/s12565-018-0462-x
pii: 10.1007/s12565-018-0462-x
doi:
Substances chimiques
Calcium Channel Blockers
0
Food Preservatives
0
Intermediate Filament Proteins
0
Keratin-17
0
Keratin-5
0
Nisin
1414-45-5
Bepridil
755BO701MA
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
163-171Subventions
Organisme : JSPS KAKENHI Grant
ID : 15K21564
Organisme : JSPS KAKENHI Grant
ID : 17K18302
Organisme : Fukuoka Dental College (JP)
ID : Regenerative Medicine Center public research funding
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