Genomic 3D compartments emerge from unfolding mitotic chromosomes.


Journal

Chromosoma
ISSN: 1432-0886
Titre abrégé: Chromosoma
Pays: Austria
ID NLM: 2985138R

Informations de publication

Date de publication:
03 2019
Historique:
received: 14 06 2018
accepted: 07 10 2018
revised: 04 10 2018
pubmed: 26 10 2018
medline: 9 8 2019
entrez: 26 10 2018
Statut: ppublish

Résumé

The 3D organisation of the genome in interphase cells is not a randomly folded polymer. Rather, experiments show that chromosomes arrange into a network of 3D compartments that correlate with biological processes, such as transcription, chromatin modifications and protein binding. However, these compartments do not exist during cell division when the DNA is condensed, and it is unclear how and when they emerge. In this paper, we focus on the early stages after cell division as the chromosomes start to decondense. We use a simple polymer model to understand the types of 3D structures that emerge from local unfolding of a compact initial state. From simulations, we recover 3D compartments, such as TADs and A/B compartments that are consistently detected in chromosome capture experiments across cell types and organisms. This suggests that the large-scale 3D organisation is a result of an inflation process.

Identifiants

pubmed: 30357462
doi: 10.1007/s00412-018-0684-7
pii: 10.1007/s00412-018-0684-7
pmc: PMC6394678
doi:

Substances chimiques

DNA 9007-49-2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Pagination

15-20

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Auteurs

Rajendra Kumar (R)

Integrated Science Lab, Umeå University, Umeå, Sweden.
Department of Physics, Umeå University, Umeå, Sweden.

Ludvig Lizana (L)

Integrated Science Lab, Umeå University, Umeå, Sweden. ludvig.lizana@umu.se.
Department of Physics, Umeå University, Umeå, Sweden. ludvig.lizana@umu.se.

Per Stenberg (P)

Department of Ecology and Environmental Science (EMG), Umeå University, Umeå, Sweden. per.stenberg@umu.se.
Division of CBRN Security and Defence, FOI-Swedish Defence Research Agency, Umeå, Sweden. per.stenberg@umu.se.

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Classifications MeSH