Klotho modulates ER-mediated signaling crosstalk between prosurvival autophagy and apoptotic cell death during LPS challenge.
Apoptosis
/ drug effects
Autophagy
/ drug effects
Bacterial Infections
/ genetics
Cell Death
/ drug effects
Cell Survival
/ drug effects
Cells, Cultured
Endoplasmic Reticulum
/ drug effects
Endoplasmic Reticulum Stress
/ drug effects
Glucuronidase
/ physiology
Humans
Inflammation
/ chemically induced
Klotho Proteins
Lipopolysaccharides
Receptor Cross-Talk
/ drug effects
Signal Transduction
/ drug effects
Wound Healing
/ drug effects
Autophagy
ER stress
Fibroblasts
Klotho
LPS
Journal
Apoptosis : an international journal on programmed cell death
ISSN: 1573-675X
Titre abrégé: Apoptosis
Pays: Netherlands
ID NLM: 9712129
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
pubmed:
26
10
2018
medline:
21
4
2020
entrez:
26
10
2018
Statut:
ppublish
Résumé
Bacterial endotoxins have been shown to induce prosurvival autophagy or apoptosis in fibroblasts and thus impair the wound healing process. Endoplasmic reticulum has been proposed as a molecular switch between these processes and klotho protein possessing pleiotropic characteristics seems to be involved in both processes, however the exact molecular mechanism is unknown. In this study, we have evaluated the effect of klotho silencing on human fibroblasts exposed to a non-toxic dose of lipopolysaccharide in terms of in vitro wound healing ability. We show for the first time, that klotho silencing in fibroblasts intensified lipopolysaccharide-induced oxidative stress and inflammatory response, what resulted in genomic instability, p-eIF2a-mediated ER stress, retardation of prosurvival autophagy, induction of apoptotic cell death and finally in impaired wound closure. Therefore, our data suggest that klotho serves as a part of cellular defense mechanism engaged in providing protection against bacterial infections during wound healing by modulating ER-signaling crosstalk between autophagy and apoptosis.
Identifiants
pubmed: 30357572
doi: 10.1007/s10495-018-1496-1
pii: 10.1007/s10495-018-1496-1
doi:
Substances chimiques
Lipopolysaccharides
0
Glucuronidase
EC 3.2.1.31
Klotho Proteins
EC 3.2.1.31
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
95-107Références
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