Post-translational Assembly of Protein Parts into Complex Devices by Using SpyTag/SpyCatcher Protein Ligase.
SpyTag/SpyCatcher
post-translational protein assembly
protein engineering
synthetic biology
Journal
Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360
Informations de publication
Date de publication:
01 02 2019
01 02 2019
Historique:
received:
11
09
2018
pubmed:
26
10
2018
medline:
28
11
2019
entrez:
26
10
2018
Statut:
ppublish
Résumé
Exploiting the innate modularity of proteins has allowed advances across the fields of synthetic biology and biotechnology. By using standardized protein components as building blocks, complex, multiprotein assemblies with sophisticated functions can be generated; feats previously not possible with strictly genetic-engineering approaches. The development of strategies for protein assembly is accelerating, pushing the boundaries of protein architecture. SpyTag and SpyCatcher protein ligase is a recent advance in this field that allows plug-and-play modularity by harnessing post-translational protein assembly. Herein, we review the latest applications of this powerful tool including novel enzyme assemblies, modularizing protein display, and the generation of antibody and antibody-like "devices" by using SpyTag/SpyCatcher technology.
Identifiants
pubmed: 30358052
doi: 10.1002/cbic.201800538
doi:
Substances chimiques
Ligases
EC 6.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
319-328Informations de copyright
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.