Osteopontin protects against pneumococcal infection in a murine model of allergic airway inflammation.
Animals
Asthma
/ chemically induced
Bacterial Load
/ drug effects
Bronchoalveolar Lavage Fluid
/ chemistry
Disease Models, Animal
Humans
Inflammation
/ chemically induced
Lung Injury
/ drug therapy
Mice
Mice, Knockout
Osteopontin
/ genetics
Pneumococcal Infections
/ drug therapy
Protective Agents
/ pharmacology
Pyroglyphidae
/ immunology
Streptococcus pneumoniae
allergy
asthma
host defense
house dust mite
osteopontin
Journal
Allergy
ISSN: 1398-9995
Titre abrégé: Allergy
Pays: Denmark
ID NLM: 7804028
Informations de publication
Date de publication:
04 2019
04 2019
Historique:
received:
21
06
2018
revised:
17
08
2018
accepted:
07
09
2018
pubmed:
27
10
2018
medline:
6
5
2020
entrez:
27
10
2018
Statut:
ppublish
Résumé
In atopic asthma, chronic Th2-biased inflammation is associated with an increased risk of pneumococcal infection. The anionic phosphoglycoprotein osteopontin (OPN) is highly expressed in asthma and has been ascribed several roles during inflammation. This study aimed to investigate whether OPN affects inflammation and vulnerability to pneumococcal infection in atopic asthma. House dust mite (HDM) extract was used to induce allergic airway inflammation in both wild-type (Spp1 Both allergen challenge in individuals with allergic asthma and the intranasal instillation of HDM in mice resulted in increased OPN levels in bronchoalveolar lavage fluid (BALF). More immune cells (including alveolar macrophages, neutrophils, eosinophils, and lymphocytes) and higher levels of proinflammatory cytokines were found in Spp1 OPN reduces inflammation, decreases tissue injury, and reduces bacterial loads during concurrent pneumococcal infection and allergic airway inflammation in a murine model. These findings suggest that OPN significantly affects vulnerability to pneumococcal infection in atopic asthma.
Sections du résumé
BACKGROUND
In atopic asthma, chronic Th2-biased inflammation is associated with an increased risk of pneumococcal infection. The anionic phosphoglycoprotein osteopontin (OPN) is highly expressed in asthma and has been ascribed several roles during inflammation. This study aimed to investigate whether OPN affects inflammation and vulnerability to pneumococcal infection in atopic asthma.
METHODS
House dust mite (HDM) extract was used to induce allergic airway inflammation in both wild-type (Spp1
RESULTS
Both allergen challenge in individuals with allergic asthma and the intranasal instillation of HDM in mice resulted in increased OPN levels in bronchoalveolar lavage fluid (BALF). More immune cells (including alveolar macrophages, neutrophils, eosinophils, and lymphocytes) and higher levels of proinflammatory cytokines were found in Spp1
CONCLUSION
OPN reduces inflammation, decreases tissue injury, and reduces bacterial loads during concurrent pneumococcal infection and allergic airway inflammation in a murine model. These findings suggest that OPN significantly affects vulnerability to pneumococcal infection in atopic asthma.
Substances chimiques
Protective Agents
0
Spp1 protein, mouse
0
Osteopontin
106441-73-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
663-674Subventions
Organisme : Swedish Heart and Lung Foundation
Pays : International
Organisme : Swedish Government Funds for Clinical Research (ALF)
Pays : International
Organisme : Swedish Foundation for Strategic Research
Pays : International
Organisme : Åke Wiberg Foundation
Pays : International
Organisme : Alfred Österlund Foundation
Pays : International
Organisme : Swedish Research Council
Pays : International
Informations de copyright
© 2018 EAACI and John Wiley and Sons A/S. Published by John Wiley and Sons Ltd.