"Normal values" for the lymphocyte cytokinesis-block micronucleus cytome parameters: Repeatability and reproducibility in a healthy reference population.
Biomonitoring
Chromosomal instability
Cytome assay
Healthy subjects
Micronucleus
Occupational exposure
Journal
The Science of the total environment
ISSN: 1879-1026
Titre abrégé: Sci Total Environ
Pays: Netherlands
ID NLM: 0330500
Informations de publication
Date de publication:
20 Feb 2019
20 Feb 2019
Historique:
received:
08
06
2018
revised:
08
10
2018
accepted:
13
10
2018
pubmed:
28
10
2018
medline:
8
2
2019
entrez:
28
10
2018
Statut:
ppublish
Résumé
The micronucleus test in peripheral blood lymphocytes is the most widely validated technique to evaluate the DNA damage and chromosomal instability in human populations. The test is largely applied in monitoring environmental and occupational exposure to genotoxic agents. It was also proposed as a biomarker of risk/susceptibility for cancer and other degenerative diseases. The availability of "normal values" in healthy populations is a main requisite for the assay application in human biomonitoring. Age and gender-related ranges of micronucleated binucleated cells (MNBN) baseline values were established in a group of 103 healthy platelet donors (50 males and 53 females) not recently exposed to genotoxic agents and characterized for demographic, lifestyle and dietary factors. Repeatability of the test by the same scorer was evaluated. Reproducibility was estimated through analysis of repeated blood samples. High correlation between the results of the three blood samplings in two separate scoring sessions of MNBN/1000BN (R
Identifiants
pubmed: 30368181
pii: S0048-9697(18)34078-6
doi: 10.1016/j.scitotenv.2018.10.187
pii:
doi:
Substances chimiques
Biomarkers
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
513-522Informations de copyright
Copyright © 2018 Elsevier B.V. All rights reserved.