Simple PDMS microdevice for biomedical applications.

Biomarkers / analysis DNA / analysis Dimethylpolysiloxanes / chemistry Genotyping Techniques / instrumentation Humans Lab-On-A-Chip Devices MicroRNAs / analysis Multiplex Polymerase Chain Reaction / instrumentation Real-Time Polymerase Chain Reaction / instrumentation Staphylococcus aureus / genetics Streptococcus pneumoniae / genetics
Bio-functional surface On-chip genetic analysis On-chip microRNA analysis On-chip microRNA purification PDMS microdevice

Journal

Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R

Informations de publication

Date de publication:
01 Feb 2019
Historique:
received: 18 09 2018
revised: 19 09 2018
accepted: 21 09 2018
entrez: 29 10 2018
pubmed: 29 10 2018
medline: 14 11 2018
Statut: ppublish

Résumé

Polydimethylsiloxane (PDMS) is a well-known biocompatible polymer employed for many applications in the biomedical field. In this study, the biocompatibility and versatility of PDMS was tested setting up a microdevice devoted to the purification and analysis of nucleic acids. The PDMS microdevice was demonstrated to successfully fulfill all requirements of genetic analyses such as genotyping and pathogen DNA identification both in multiplex and real-time PCR, suggesting the possibility to carry out a molecular test directly on-chip. Moreover, the PDMS microdevice was successfully applied to the purification and detection of disease biomarkers, such as microRNAs related to cancer or heart disease. On-chip microRNA purification was demonstrated starting from clinically relevant samples, i.e. plasma, serum, tissue biopsies. Significantly, the purification and the transcription of microRNA into cDNA occur in the same PDMS chamber, saving time and labor for the overall analysis. Again, the PDMS microdevice was confirmed as a notable candidate for compact, rapid, easy-to-use molecular tests.

Identifiants

DOI: 10.1016/j.talanta.2018.09.080 PMID: 30368296
pubmed: 30368296
pii: S0039-9140(18)30996-2
doi: 10.1016/j.talanta.2018.09.080
pii:
doi:

Substances chimiques

Biomarkers 0
Dimethylpolysiloxanes 0
MicroRNAs 0
baysilon 63148-62-9
DNA 9007-49-2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

44-50

Informations de copyright

Copyright © 2018 Elsevier B.V. All rights reserved.

Auteurs

C Potrich (C)

Fondazione Bruno Kessler, Center for Materials and Microsystems, Lab. Biomarker Studies and Structure Analysis for Health, via Sommarive 18, I-38123 Povo (Trento), Italy; CNR - Consiglio Nazionale delle Ricerche, Istituto di Biofisica, via alla Cascata 56/C, I-38123, Povo (Trento), Italy. Electronic address: cpotrich@fbk.eu.

L Lunelli (L)

Fondazione Bruno Kessler, Center for Materials and Microsystems, Lab. Biomarker Studies and Structure Analysis for Health, via Sommarive 18, I-38123 Povo (Trento), Italy; CNR - Consiglio Nazionale delle Ricerche, Istituto di Biofisica, via alla Cascata 56/C, I-38123, Povo (Trento), Italy.

M Cocuzza (M)

Department of Applied Science and Technology, Politecnico di Torino, Corso Duca degli Abruzzi 24, Torino I-10129, Italy; CNR-IMEM, Parco Area delle Scienze 37a, 43124 Parma, Italy.

S L Marasso (SL)

Department of Applied Science and Technology, Politecnico di Torino, Corso Duca degli Abruzzi 24, Torino I-10129, Italy; CNR-IMEM, Parco Area delle Scienze 37a, 43124 Parma, Italy.

C F Pirri (CF)

Department of Applied Science and Technology, Politecnico di Torino, Corso Duca degli Abruzzi 24, Torino I-10129, Italy.

C Pederzolli (C)

Fondazione Bruno Kessler, Center for Materials and Microsystems, Lab. Biomarker Studies and Structure Analysis for Health, via Sommarive 18, I-38123 Povo (Trento), Italy.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Effectiveness of Virtual Yoga for Chronic Low Back Pain: A Randomized Clinical Trial.

Hallie Tankha, Devyn Gaskins, Amanda Shallcross et al.
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH

questionsmedicales.fr › Facteurs biologiques › Marqueurs biologiques › Simple PDMS microdevice for biomedical applications.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › Simple PDMS microdevice for biomedical applications.
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Siloxanes › Silicone › Polydiméthylsiloxanes › Simple PDMS microdevice for biomedical applications.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques de génotypage › Simple PDMS microdevice for biomedical applications.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Simple PDMS microdevice for biomedical applications.
questionsmedicales.fr › Équipement et fournitures › Équipement et fournitures électriques › Laboratoires sur puces › Simple PDMS microdevice for biomedical applications.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › microARN › Simple PDMS microdevice for biomedical applications.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine multiplex › Simple PDMS microdevice for biomedical applications.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine en temps réel › Simple PDMS microdevice for biomedical applications.
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Cocci à Gram positif › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Simple PDMS microdevice for biomedical applications.
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pneumoniae › Simple PDMS microdevice for biomedical applications.