Crystallographic evidence for two-metal-ion catalysis in human pol η.
polymerases
polymerization
pyrophosphorolysis
saveED
spherically averaged electron density function
three-metal-ion catalysis
two-metal-ion catalysis
Journal
Protein science : a publication of the Protein Society
ISSN: 1469-896X
Titre abrégé: Protein Sci
Pays: United States
ID NLM: 9211750
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
20
08
2018
revised:
01
10
2018
accepted:
02
10
2018
pubmed:
29
10
2018
medline:
25
1
2020
entrez:
29
10
2018
Statut:
ppublish
Résumé
Extensive evidence exists that DNA polymerases use two metal ions to catalyze the phosphoryl transfer reaction. Recently, competing evidence emerged, suggesting that a third metal ion, known as MnC, may be involved in catalysis. The binding of MnC was observed in crystal structures of the replication complexes of human polymerase (pol) η, pol β, and pol μ. Its occupancy (q
Identifiants
pubmed: 30368948
doi: 10.1002/pro.3541
pmc: PMC6319759
doi:
Substances chimiques
DNA-Directed DNA Polymerase
EC 2.7.7.7
POLN protein, human
EC 2.7.7.7
Magnesium
I38ZP9992A
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
439-447Subventions
Organisme : NIGMS NIH HHS
ID : P01 GM022778
Pays : United States
Organisme : NIH HHS
ID : GM022778
Pays : United States
Informations de copyright
© 2018 The Protein Society.
Références
Science. 1994 Jun 24;264(5167):1891-903
pubmed: 7516580
J Biol Chem. 2011 Feb 4;286(5):3755-66
pubmed: 21084297
Biochemistry. 2001 Jul 31;40(30):9014-22
pubmed: 11467964
Biochemistry. 2009 Mar 17;48(10):2075-86
pubmed: 19228037
Methods. 1999 May;18(1):38-50
pubmed: 10208815
Nucleic Acids Res. 2017 Mar 17;45(5):2736-2745
pubmed: 28108654
Science. 1998 Jan 16;279(5349):356-8
pubmed: 9430581
Biochemistry. 1992 Nov 17;31(45):10984-94
pubmed: 1332748
Structure. 2006 Apr;14(4):757-66
pubmed: 16615916
Cell. 2001 Jun 1;105(5):657-67
pubmed: 11389835
Nat Commun. 2017 Aug 15;8(1):253
pubmed: 28811466
Biochemistry. 1987 Dec 15;26(25):8410-7
pubmed: 3327522
Biochemistry. 2009 Mar 17;48(10):2087-98
pubmed: 19228036
Protein Sci. 2017 May;26(5):1012-1023
pubmed: 28244185
Science. 2016 Jun 10;352(6291):1334-7
pubmed: 27284197
Inorg Chem. 2017 Jan 3;56(1):313-320
pubmed: 27976880
Nature. 1998 Jan 15;391(6664):251-8
pubmed: 9440688
Science. 1998 Nov 27;282(5394):1669-75
pubmed: 9831551
Proc Natl Acad Sci U S A. 2015 Sep 22;112(38):E5228-36
pubmed: 26351676
Proc Natl Acad Sci U S A. 2007 Mar 13;104(11):4267-72
pubmed: 17360513
Acta Crystallogr D Biol Crystallogr. 1997 May 1;53(Pt 3):240-55
pubmed: 15299926
Nature. 2012 Jul 11;487(7406):196-201
pubmed: 22785315
Mol Cell. 2006 Apr 7;22(1):5-13
pubmed: 16600865
Biochemistry. 2011 Oct 25;50(42):9114-24
pubmed: 21923197
Methods Enzymol. 2009;468:3-30
pubmed: 20946762
Protein Sci. 2018 Feb;27(2):411-420
pubmed: 29027293
Biochemistry. 2005 Apr 5;44(13):5177-87
pubmed: 15794655
Science. 2003 Mar 28;299(5615):2067-71
pubmed: 12637673
Curr Opin Struct Biol. 1998 Dec;8(6):704-12
pubmed: 9914251
Biochim Biophys Acta. 2010 May;1804(5):1041-8
pubmed: 20079883
Methods Mol Biol. 2012;848:275-96
pubmed: 22315075
Science. 1988 Oct 28;242(4878):533-40
pubmed: 3051385
Acc Chem Res. 2017 Jan 17;50(1):41-48
pubmed: 28001034
Cell. 2013 Jul 3;154(1):157-68
pubmed: 23827680
ACS Energy Lett. 2017 Feb 10;2(2):397-407
pubmed: 28217747
Proteins. 2017 Aug;85(8):1446-1453
pubmed: 28383109
Nat Struct Mol Biol. 2009 Feb;16(2):212-8
pubmed: 19151724
J Biol Chem. 1968 Feb 10;243(3):627-38
pubmed: 4866523
EMBO J. 2006 Aug 9;25(15):3458-68
pubmed: 16900098
Biochemistry. 1991 Jan 15;30(2):511-25
pubmed: 1846298