Establishment of widely applicable DNA extraction methods to identify the origins of crude drugs derived from animals using molecular techniques.

BLAST analysis Crude drugs DNA extraction Mitochondrial 16S rRNA gene Origin identification

Journal

Journal of natural medicines
ISSN: 1861-0293
Titre abrégé: J Nat Med
Pays: Japan
ID NLM: 101518405

Informations de publication

Date de publication:
Jan 2019
Historique:
received: 02 07 2018
accepted: 17 10 2018
pubmed: 31 10 2018
medline: 1 6 2019
entrez: 31 10 2018
Statut: ppublish

Résumé

We established widely applicable DNA extraction methods to identify the origins of crude drugs derived from animals. Twenty-one samples including 17 kinds of crude drug derived from animals were examined. DNA was extracted from most of the crude drugs by adjustment of the QIAamp

Identifiants

pubmed: 30374697
doi: 10.1007/s11418-018-1261-3
pii: 10.1007/s11418-018-1261-3
doi:

Substances chimiques

Complex Mixtures 0
DNA, Bacterial 0
Pharmaceutical Preparations 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

173-178

Subventions

Organisme : Japan Society for the Promotion of Science (JP)
ID : JP16K08304

Références

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Auteurs

Hiroaki Nakanishi (H)

Department of Forensic Medicine, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-Ku, Tokyo, 113-8421, Japan. hnakani@juntendo.ac.jp.

Katsumi Yoneyama (K)

Department of Forensic Medicine, Saitama Medical University, 38 Morohongo, Moroyama, Saitama, 350-0495, Japan.

Yoshie Hayashizaki (Y)

Department of Forensic Medicine, Saitama Medical University, 38 Morohongo, Moroyama, Saitama, 350-0495, Japan.

Masaaki Hara (M)

Department of Forensic Medicine, Saitama Medical University, 38 Morohongo, Moroyama, Saitama, 350-0495, Japan.

Aya Takada (A)

Department of Forensic Medicine, Saitama Medical University, 38 Morohongo, Moroyama, Saitama, 350-0495, Japan.

Kazuyuki Saito (K)

Department of Forensic Medicine, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-Ku, Tokyo, 113-8421, Japan.

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Classifications MeSH