Integrated generation of induced pluripotent stem cells in a low-cost device.

Alginates / chemistry Biocompatible Materials / chemistry Cell Differentiation Cell Line Cell Proliferation Cellular Reprogramming Equipment Design Humans Induced Pluripotent Stem Cells / cytology Tissue Engineering / economics
Alginate hydrogel tubes Human induced pluripotent stem cells Personalized medicine Reprogramming Single-conical-tube device

Journal

Biomaterials
ISSN: 1878-5905
Titre abrégé: Biomaterials
Pays: Netherlands
ID NLM: 8100316

Informations de publication

Date de publication:
01 2019
Historique:
received: 07 08 2018
revised: 16 10 2018
accepted: 19 10 2018
pubmed: 2 11 2018
medline: 7 3 2020
entrez: 2 11 2018
Statut: ppublish

Résumé

Human induced pluripotent stem cells (iPSCs) have unlimited proliferation capability and potential to differentiate into all somatic cells. Their derivatives contain patients' genetic information and can model many diseases. Additionally, derivatives of patient-specific iPSCs induce minimal immune rejection in vivo. With this unique combination of properties, iPSCs open the avenue to personalized medicine including personalized drug screening, toxicity test, cell therapy and tissue engineering. However, the further advance of iPSC-based personalized medicine is currently limited by the difficulty to generate iPSCs for large populations and at affordable cost. We here report a low-cost device to address this challenge. The device allows the entire bioprocess for generating high quality and quantity of iPSCs for one patient to be done automatically within a closed conical tube without cell passaging. Additionally, iPSCs can be further differentiated into somatic cells in the device. Thus, the device also allows integrated iPSCs generation, expansion and differentiation to produce any somatic cell types. This device can be made in large quantities at low cost for manufacturing iPSCs (and their derivatives in necessary) for large populations at affordable cost. It will significantly advance the iPSCs-based personalized medicine.

Identifiants

DOI: 10.1016/j.biomaterials.2018.10.027 PMID: 30384126
pubmed: 30384126
pii: S0142-9612(18)30741-5
doi: 10.1016/j.biomaterials.2018.10.027
pii:
doi:

Substances chimiques

Alginates 0
Biocompatible Materials 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

23-36

Informations de copyright

Copyright © 2018 Elsevier Ltd. All rights reserved.

Auteurs

Haishuang Lin (H)

Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Nebraska, USA.

Qiang Li (Q)

Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Nebraska, USA; Biomedical Engineering Program, University of Nebraska-Lincoln, Nebraska, USA.

Qian Du (Q)

Department of Biological Systems Engineering, University of Nebraska-Lincoln, Nebraska, USA.

Ou Wang (O)

Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Nebraska, USA; Biomedical Engineering Program, University of Nebraska-Lincoln, Nebraska, USA.

Zhanqi Wang (Z)

Department of Vascular Surgery, Beijing Anzhen Hospital of Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing, China.

Leonard Akert (L)

Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Nebraska, USA.

Mark A Carlson (MA)

Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Nebraska, USA; VA Nebraska Western-Iowa Health Care System, VA Medical Center, Nebraska, USA.

Chi Zhang (C)

Department of Biological Systems Engineering, University of Nebraska-Lincoln, Nebraska, USA.

Anuradha Subramanian (A)

Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Nebraska, USA.

Chi Zhang (C)

Department of Radiation Oncology, College of Medicine, University of Nebraska Medical Center, Omaha, NE, USA.

Matthew Lunning (M)

Division of Oncology and Hematology, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, NE, USA.

Ming Li (M)

Department of Psychology, University of Nebraska-Lincoln, Nebraska, USA.

Yuguo Lei (Y)

Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Nebraska, USA; Biomedical Engineering Program, University of Nebraska-Lincoln, Nebraska, USA; Mary and Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE, USA; Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE, USA. Electronic address: ylei14@unl.edu.

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Classifications MeSH

questionsmedicales.fr › Glucides › Polyosides › Alginates › Integrated generation of induced pluripotent...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Matériaux biocompatibles › Integrated generation of induced pluripotent...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Integrated generation of induced pluripotent...
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › Integrated generation of induced pluripotent...
questionsmedicales.fr › Phénomènes physiologiques › Croissance et développement › Croissance › Processus de croissance cellulaire › Prolifération cellulaire › Integrated generation of induced pluripotent...
questionsmedicales.fr › Phénomènes génétiques › Reprogrammation cellulaire › Integrated generation of induced pluripotent...
questionsmedicales.fr › Techniques d'investigation › Conception d'appareillage › Integrated generation of induced pluripotent...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Integrated generation of induced pluripotent...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Cellules souches pluripotentes induites › Integrated generation of induced pluripotent...
questionsmedicales.fr › Technologie, industrie et agriculture › Ingénierie › Bioingénierie › Ingénierie cellulaire › Ingénierie tissulaire › Integrated generation of induced pluripotent...