Inflammation and vascular permeability correlate with growth in sporadic vestibular schwannoma.


Journal

Neuro-oncology
ISSN: 1523-5866
Titre abrégé: Neuro Oncol
Pays: England
ID NLM: 100887420

Informations de publication

Date de publication:
19 02 2019
Historique:
pubmed: 6 11 2018
medline: 6 5 2020
entrez: 3 11 2018
Statut: ppublish

Résumé

Inflammation is hypothesized to be a key event in the growth of sporadic vestibular schwannoma (VS). In this study we sought to investigate the relationship between inflammation and tumor growth in vivo using the PET tracer 11C-(R)-PK11195 and dynamic contrast enhanced (DCE) MRI derived vascular biomarkers. Nineteen patients with sporadic VS (8 static, 7 growing, and 4 shrinking tumors) underwent prospective imaging with dynamic 11C-(R)-PK11195 PET and a comprehensive MR protocol, including high temporal resolution DCE-MRI in 15 patients. An intertumor comparison of 11C-(R)-PK11195 binding potential (BPND) and DCE-MRI derived vascular biomarkers (Ktrans, vp, ve) across the 3 different tumor growth cohorts was undertaken. Tissue of 8 tumors was examined with immunohistochemistry markers for inflammation (Iba1), neoplastic cells (S-100 protein), vessels (CD31), the PK11195 target translocator protein (TSPO), fibrinogen for vascular permeability, and proliferation (Ki-67). Results were correlated with PET and DCE-MRI data. Compared with static tumors, growing VS displayed significantly higher mean 11C-(R)-PK11195 BPND (-0.07 vs 0.47, P = 0.020), and higher mean tumor Ktrans (0.06 vs 0.14, P = 0.004). Immunohistochemistry confirmed the imaging findings and demonstrated that TSPO is predominantly expressed in macrophages. Within growing VS, macrophages rather than tumor cells accounted for the majority of proliferating cells. We present the first in vivo imaging evidence of increased inflammation within growing sporadic VS. Our results demonstrate that 11C-(R)-PK11195 specific binding and DCE-MRI derived parameters can be used as imaging biomarkers of inflammation and vascular permeability in this tumor group.

Sections du résumé

BACKGROUND
Inflammation is hypothesized to be a key event in the growth of sporadic vestibular schwannoma (VS). In this study we sought to investigate the relationship between inflammation and tumor growth in vivo using the PET tracer 11C-(R)-PK11195 and dynamic contrast enhanced (DCE) MRI derived vascular biomarkers.
METHODS
Nineteen patients with sporadic VS (8 static, 7 growing, and 4 shrinking tumors) underwent prospective imaging with dynamic 11C-(R)-PK11195 PET and a comprehensive MR protocol, including high temporal resolution DCE-MRI in 15 patients. An intertumor comparison of 11C-(R)-PK11195 binding potential (BPND) and DCE-MRI derived vascular biomarkers (Ktrans, vp, ve) across the 3 different tumor growth cohorts was undertaken. Tissue of 8 tumors was examined with immunohistochemistry markers for inflammation (Iba1), neoplastic cells (S-100 protein), vessels (CD31), the PK11195 target translocator protein (TSPO), fibrinogen for vascular permeability, and proliferation (Ki-67). Results were correlated with PET and DCE-MRI data.
RESULTS
Compared with static tumors, growing VS displayed significantly higher mean 11C-(R)-PK11195 BPND (-0.07 vs 0.47, P = 0.020), and higher mean tumor Ktrans (0.06 vs 0.14, P = 0.004). Immunohistochemistry confirmed the imaging findings and demonstrated that TSPO is predominantly expressed in macrophages. Within growing VS, macrophages rather than tumor cells accounted for the majority of proliferating cells.
CONCLUSION
We present the first in vivo imaging evidence of increased inflammation within growing sporadic VS. Our results demonstrate that 11C-(R)-PK11195 specific binding and DCE-MRI derived parameters can be used as imaging biomarkers of inflammation and vascular permeability in this tumor group.

Identifiants

pubmed: 30388263
pii: 5155625
doi: 10.1093/neuonc/noy177
pmc: PMC6380424
doi:

Substances chimiques

AIF1 protein, human 0
Calcium-Binding Proteins 0
Carbon Radioisotopes 0
Carbon-11 0
Isoquinolines 0
Ki-67 Antigen 0
MKI67 protein, human 0
Microfilament Proteins 0
PECAM1 protein, human 0
Platelet Endothelial Cell Adhesion Molecule-1 0
Receptors, GABA 0
S100 Proteins 0
TSPO protein, human 0
Fibrinogen 9001-32-5
PK 11195 YNF83VN1RL

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

314-325

Informations de copyright

© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Neuro-Oncology.

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Auteurs

Daniel Lewis (D)

Manchester Centre for Clinical Neurosciences, Salford Royal NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.
Wolfson Molecular Imaging Centre, Division of Informatics, Imaging and Data Sciences, University of Manchester, Manchester, UK.

Federico Roncaroli (F)

Manchester Centre for Clinical Neurosciences, Salford Royal NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.
Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.

Erjon Agushi (E)

Manchester Centre for Clinical Neurosciences, Salford Royal NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.
Wolfson Molecular Imaging Centre, Division of Informatics, Imaging and Data Sciences, University of Manchester, Manchester, UK.

Dominic Mosses (D)

Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.

Ricky Williams (R)

Brain Tumour Biobank, Salford Royal NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.

Ka-Loh Li (KL)

Wolfson Molecular Imaging Centre, Division of Informatics, Imaging and Data Sciences, University of Manchester, Manchester, UK.

Xiaoping Zhu (X)

Wolfson Molecular Imaging Centre, Division of Informatics, Imaging and Data Sciences, University of Manchester, Manchester, UK.

Rainer Hinz (R)

Wolfson Molecular Imaging Centre, Division of Informatics, Imaging and Data Sciences, University of Manchester, Manchester, UK.

Ross Atkinson (R)

Manchester Centre for Clinical Neurosciences, Salford Royal NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.

Andrea Wadeson (A)

Manchester Skull Base Unit, Manchester Centre for Clinical Neurosciences, Salford Royal NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.

Sharon Hulme (S)

Manchester Centre for Clinical Neurosciences, Salford Royal NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.

Helen Mayers (H)

Department of Cellular Pathology, Salford Royal NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.

Emma Stapleton (E)

Manchester Skull Base Unit, Manchester Centre for Clinical Neurosciences, Salford Royal NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.

Simon K L Lloyd (SKL)

Manchester Skull Base Unit, Manchester Centre for Clinical Neurosciences, Salford Royal NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.

Simon R Freeman (SR)

Manchester Skull Base Unit, Manchester Centre for Clinical Neurosciences, Salford Royal NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.

Scott A Rutherford (SA)

Manchester Skull Base Unit, Manchester Centre for Clinical Neurosciences, Salford Royal NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.

Charlotte Hammerbeck-Ward (C)

Manchester Skull Base Unit, Manchester Centre for Clinical Neurosciences, Salford Royal NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.

D Gareth Evans (DG)

Manchester Centre for Genomic Medicine, St Mary's Hospital, Manchester University Hospitals National Health Service Foundation Trust and Manchester Academic Health Science Centre, Manchester, UK.

Omar Pathmanaban (O)

Manchester Skull Base Unit, Manchester Centre for Clinical Neurosciences, Salford Royal NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.

Alan Jackson (A)

Wolfson Molecular Imaging Centre, Division of Informatics, Imaging and Data Sciences, University of Manchester, Manchester, UK.

Andrew T King (AT)

Manchester Centre for Clinical Neurosciences, Salford Royal NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.
Manchester Skull Base Unit, Manchester Centre for Clinical Neurosciences, Salford Royal NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.
Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.

David J Coope (DJ)

Manchester Centre for Clinical Neurosciences, Salford Royal NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.
Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.

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