Reductive nitrosylation of ferric microperoxidase-11.


Journal

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry
ISSN: 1432-1327
Titre abrégé: J Biol Inorg Chem
Pays: Germany
ID NLM: 9616326

Informations de publication

Date de publication:
02 2019
Historique:
received: 16 05 2018
accepted: 08 10 2018
pubmed: 6 11 2018
medline: 26 11 2019
entrez: 4 11 2018
Statut: ppublish

Résumé

Microperoxidase-11 (MP11) is an undecapeptide derived from horse heart cytochrome c, which is considered as a heme-protein model. Here, the reductive nitrosylation of ferric MP11 (MP11(III)) under anaerobic conditions has been investigated between pH 7.4 and 9.2, at T = 20.0 °C. At pH ≤ 7.7, NO binds reversibly to MP11(III) leading to the formation of the MP11(III)-NO complex. However, between pH 8.2 and 9.2, the addition of NO to MP11(III) leads to the formation of ferrous nitrosylated MP11(II) (MP11(II)-NO). In fact, the transient MP11{FeNO}

Identifiants

pubmed: 30390140
doi: 10.1007/s00775-018-1623-z
pii: 10.1007/s00775-018-1623-z
doi:

Substances chimiques

Cytochrome c Group 0
Iron Compounds 0
Nitroso Compounds 0
Peroxidases EC 1.11.1.-
microperoxidase EC 1.11.1.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

21-29

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Auteurs

Paolo Ascenzi (P)

Interdepartmental Laboratory of Electron Microscopy, Roma Tre University, Via della Vasca Navale 79, 00146, Rome, Italy. ascenzi@uniroma3.it.

Giovanna De Simone (G)

Department of Sciences, Roma Tre University, Viale Gugliemo Marconi 446, 00146, Rome, Italy.

Diego Sbardella (D)

Department of Clinical Sciences and Translational Medicine, University of Roma "Tor Vergata", Via Montpellier 1, 00133, Rome, Italy.
Interuniversity Consortium for the Research on the Chemistry of Metals in Biological Systems, Via Celso Ulpiani 27, 70126, Bari, Italy.

Massimo Coletta (M)

Department of Clinical Sciences and Translational Medicine, University of Roma "Tor Vergata", Via Montpellier 1, 00133, Rome, Italy.
Interuniversity Consortium for the Research on the Chemistry of Metals in Biological Systems, Via Celso Ulpiani 27, 70126, Bari, Italy.

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Classifications MeSH