Generating Embryonic Salivary Gland Organoids.
mesenchyme
organoid
proacinar
salivary
Journal
Current protocols in cell biology
ISSN: 1934-2616
Titre abrégé: Curr Protoc Cell Biol
Pays: United States
ID NLM: 101287856
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
pubmed:
6
11
2018
medline:
7
5
2020
entrez:
6
11
2018
Statut:
ppublish
Résumé
Organoids are important research tools for studying organ morphogenesis and differentiation because they recapitulate ex vivo the native 3D organization of cells that is essential for proper cell and organ function. The composition of organoids can be manipulated to incorporate specific cell types to facilitate molecular interrogation of cell-cell interactions during organoid formation. A method for generating organoids derived from both embryonic salivary gland epithelial progenitor cells and mesenchymal support cells is described. Methods for isolating enriched populations of the epithelial cells as clusters and the mesenchyme cells as single cells from mouse embryonic submandibular salivary glands are also provided. Separating the epithelial and mesenchymal cell populations allows for independent molecular manipulation of each cell type. In addition, methods for lentiviral transduction of the mesenchyme cells and quantitative image analysis of organoids are provided. The methods described here are useful for exploring mechanisms driving organ formation. © 2018 by John Wiley & Sons, Inc.
Identifiants
pubmed: 30394683
doi: 10.1002/cpcb.76
pmc: PMC6948183
mid: NIHMS991513
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e76Subventions
Organisme : NIDCR NIH HHS
ID : R01 DE022467
Pays : United States
Organisme : NIDCR NIH HHS
ID : R01 DE027953
Pays : United States
Organisme : NIDCR NIH HHS
ID : R56 DE022467
Pays : United States
Organisme : NCRR NIH HHS
ID : C06 RR015464
Pays : United States
Organisme : NIDCR NIH HHS
ID : R21 DE027571
Pays : United States
Informations de copyright
© 2018 John Wiley & Sons, Inc.
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