Could chlorhexidine be an adequate positive control for antimicrobial photodynamic therapy in- in vitro studies?
Biofilms
/ drug effects
Child
Chlorhexidine
/ analogs & derivatives
Curcumin
/ pharmacology
Dental Caries
/ microbiology
Dentin
/ drug effects
Dose-Response Relationship, Drug
Humans
Lactobacillus
/ drug effects
Microbial Viability
/ drug effects
Photochemotherapy
/ methods
Photosensitizing Agents
/ pharmacology
Research Design
Stem Cells
Streptococcus mutans
/ drug effects
Antimicrobial photodynamic therapy
Chlorhexidine
Dental caries
Oral biofilms
Photochemotherapy
Journal
Photodiagnosis and photodynamic therapy
ISSN: 1873-1597
Titre abrégé: Photodiagnosis Photodyn Ther
Pays: Netherlands
ID NLM: 101226123
Informations de publication
Date de publication:
Mar 2019
Mar 2019
Historique:
received:
19
08
2018
revised:
25
10
2018
accepted:
02
11
2018
pubmed:
7
11
2018
medline:
18
12
2019
entrez:
7
11
2018
Statut:
ppublish
Résumé
Chlorhexidine digluconate (CHX) is commonly applied as positive control of new antimicrobials, because it is considered the gold-standard for chemical plaque control. The aim of this study was to compare the effect of treatments with curcumin-mediated aPDT and CHX in relation to the viability of specific microorganism groups in two distinct times (immediately and 24 h later). Dentin caries microcosms were grown on bovine dentin discs (37 °C, anaerobiosis) for 3 days in the Active Attachment Amsterdam Biofilm Model. The biofilms were treated with 300 μM curcumin and 75 J.cm Curcumin-mediated aPDT (C + L+), 0.06% and 0.12% CHX reduced mutans streptococci counts (0.19, 0.10 and 0.07 log10 respectively) in the immediate analysis. After 24 h, it was observed a re-growth of microorganisms treated by curcumin-mediated aPDT, whereas both CHX concentrations demonstrated a decrease of the viable microorganisms. This study confirmed the substantive effect of CHX and the immediate effect of aPDT. The use of a neutralizer solution was important to block the substantivity of CHX and permit its fair comparison with aPDT, allowing its use as a positive control in further studies.
Sections du résumé
BACKGROUND
BACKGROUND
Chlorhexidine digluconate (CHX) is commonly applied as positive control of new antimicrobials, because it is considered the gold-standard for chemical plaque control. The aim of this study was to compare the effect of treatments with curcumin-mediated aPDT and CHX in relation to the viability of specific microorganism groups in two distinct times (immediately and 24 h later).
METHODS
METHODS
Dentin caries microcosms were grown on bovine dentin discs (37 °C, anaerobiosis) for 3 days in the Active Attachment Amsterdam Biofilm Model. The biofilms were treated with 300 μM curcumin and 75 J.cm
RESULTS
RESULTS
Curcumin-mediated aPDT (C + L+), 0.06% and 0.12% CHX reduced mutans streptococci counts (0.19, 0.10 and 0.07 log10 respectively) in the immediate analysis. After 24 h, it was observed a re-growth of microorganisms treated by curcumin-mediated aPDT, whereas both CHX concentrations demonstrated a decrease of the viable microorganisms.
CONCLUSION
CONCLUSIONS
This study confirmed the substantive effect of CHX and the immediate effect of aPDT. The use of a neutralizer solution was important to block the substantivity of CHX and permit its fair comparison with aPDT, allowing its use as a positive control in further studies.
Identifiants
pubmed: 30399454
pii: S1572-1000(18)30270-9
doi: 10.1016/j.pdpdt.2018.11.004
pii:
doi:
Substances chimiques
Photosensitizing Agents
0
Curcumin
IT942ZTH98
chlorhexidine gluconate
MOR84MUD8E
Chlorhexidine
R4KO0DY52L
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
58-62Informations de copyright
Copyright © 2018 Elsevier B.V. All rights reserved.