Injectable angiogenic and osteogenic carrageenan nanocomposite hydrogel for bone tissue engineering.
Bone and Bones
/ cytology
Carrageenan
/ administration & dosage
Cell Differentiation
/ drug effects
Cell Proliferation
/ drug effects
Human Umbilical Vein Endothelial Cells
/ cytology
Humans
Hydrogels
/ chemistry
Injections
Nanocomposites
Neovascularization, Physiologic
/ drug effects
Osteogenesis
/ drug effects
Tissue Engineering
Angiogenesis
Carrageenan hydrogel
Dimethyloxallylglycine
Osteogenesis
Whitlockite nanoparticles
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
01 Feb 2019
01 Feb 2019
Historique:
received:
12
09
2018
revised:
24
10
2018
accepted:
25
10
2018
pubmed:
8
11
2018
medline:
5
4
2019
entrez:
8
11
2018
Statut:
ppublish
Résumé
Functional biomaterials that couple angiogenesis and osteogenesis processes are vital for bone tissue engineering and bone remodeling. Herein we developed an injectable carrageenan nanocomposite hydrogel incorporated with whitlockite nanoparticles and an angiogenic drug, dimethyloxallylglycine. Synthesized whitlockite nanoparticles and nanocomposite hydrogels were characterized using SEM, TEM, EDS and FTIR. Developed hydrogels were injectable, mechanically stable, cytocompatible and has better protein adsorption. Incorporation of dimethyloxallylglycine resulted in initial burst release followed by sustained release for 7 days. Human umbilical vein endothelial cells exposed to dimethyloxallylglycine incorporated nanocomposite hydrogel showed enhanced cell migration and capillary tube-like structure formation. Osteogenic differentiation in rat adipose derived mesenchymal stem cells after 7 and 14 days revealed increased levels of alkaline phosphatase activity in vitro. Furthermore, cells exposed to nanocomposite hydrogel revealed enhanced protein expressions of RUNX2, COL and OPN. Overall, these results suggest that incorporation of whitlockite and dimethyloxallylglycine in carrageenan hydrogel promoted osteogenesis and angiogenesis in vitro.
Identifiants
pubmed: 30401650
pii: S0141-8130(18)34870-0
doi: 10.1016/j.ijbiomac.2018.10.182
pii:
doi:
Substances chimiques
Hydrogels
0
Carrageenan
9000-07-1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
320-328Informations de copyright
Copyright © 2018 Elsevier B.V. All rights reserved.