Innate immune crosstalk in asthmatic airways: Innate lymphoid cells coordinate polarization of lung macrophages.
Animals
Asthma
/ immunology
Cell Differentiation
Cells, Cultured
Coculture Techniques
Cytokines
/ metabolism
Eosinophilia
/ immunology
Female
Flow Cytometry
Humans
Immunity, Innate
Lung
/ immunology
Lymphocytes
/ immunology
Macrophages, Alveolar
/ immunology
Male
Mice
Mice, Inbred C57BL
Middle Aged
Sputum
/ immunology
Th2 Cells
/ immunology
Asthma
asthma phenotype
induced sputum
innate lymphoid cells
macrophage
polarization
Journal
The Journal of allergy and clinical immunology
ISSN: 1097-6825
Titre abrégé: J Allergy Clin Immunol
Pays: United States
ID NLM: 1275002
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
15
03
2018
revised:
21
09
2018
accepted:
09
10
2018
pubmed:
12
11
2018
medline:
12
5
2020
entrez:
12
11
2018
Statut:
ppublish
Résumé
Recent studies have emphasized the role of innate lymphoid cells (ILCs) in the development of asthma. The involvement of group 2 innate lymphoid cells (ILC2s) in asthma is well studied: however, the participation of other types of ILCs in the development of asthma remains unclear. This study aims to understand the role of various ILCs in patients with asthma, especially their effect on macrophage polarization. Each subset of ILCs and macrophages in induced sputum from 51 steroid-naive patients with asthma and 18 healthy donors was analyzed by using flow cytometry. Alveolar macrophages (AM) were sorted and cocultured with each subset of ILCs to determine whether the polarization of macrophages could be regulated by ILCs. In addition to ILC2s, numbers of group 1 innate lymphoid cells (ILC1s) and group 3 innate lymphoid cells (ILC3s) were increased in induced sputum from asthmatic patients when compared with those in healthy control subjects. The dominance of macrophages in induced sputum was more prominent in asthmatic patients than in healthy control subjects. A positive correlation between numbers of ILC2s and numbers of M2 macrophages and those of ILC1s/ILC3s and M1 macrophages was observed. Coculture of ILC2s with AMs induced expression of M2 macrophage-related genes, whereas coculture of ILC1s and ILC3s with AMs induced expression of M1 macrophage-related genes through cytokine secretion, as well as cell-cell contact. According to the inflammatory signature, patients with eosinophilic asthma have more ILC2s and M2 macrophages, and those with noneosinophilic asthma have an M1 macrophage-dominant profile. A different subset of ILCs regulates macrophage polarization, contributing to developing the distinct phenotype of asthma.
Sections du résumé
BACKGROUND
Recent studies have emphasized the role of innate lymphoid cells (ILCs) in the development of asthma. The involvement of group 2 innate lymphoid cells (ILC2s) in asthma is well studied: however, the participation of other types of ILCs in the development of asthma remains unclear.
OBJECTIVE
This study aims to understand the role of various ILCs in patients with asthma, especially their effect on macrophage polarization.
METHODS
Each subset of ILCs and macrophages in induced sputum from 51 steroid-naive patients with asthma and 18 healthy donors was analyzed by using flow cytometry. Alveolar macrophages (AM) were sorted and cocultured with each subset of ILCs to determine whether the polarization of macrophages could be regulated by ILCs.
RESULTS
In addition to ILC2s, numbers of group 1 innate lymphoid cells (ILC1s) and group 3 innate lymphoid cells (ILC3s) were increased in induced sputum from asthmatic patients when compared with those in healthy control subjects. The dominance of macrophages in induced sputum was more prominent in asthmatic patients than in healthy control subjects. A positive correlation between numbers of ILC2s and numbers of M2 macrophages and those of ILC1s/ILC3s and M1 macrophages was observed. Coculture of ILC2s with AMs induced expression of M2 macrophage-related genes, whereas coculture of ILC1s and ILC3s with AMs induced expression of M1 macrophage-related genes through cytokine secretion, as well as cell-cell contact. According to the inflammatory signature, patients with eosinophilic asthma have more ILC2s and M2 macrophages, and those with noneosinophilic asthma have an M1 macrophage-dominant profile.
CONCLUSION
A different subset of ILCs regulates macrophage polarization, contributing to developing the distinct phenotype of asthma.
Identifiants
pubmed: 30414858
pii: S0091-6749(18)31582-3
doi: 10.1016/j.jaci.2018.10.040
pii:
doi:
Substances chimiques
Cytokines
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1769-1782.e11Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2018 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.