Quantification of Calcyclin and Heat Shock Protein 90 in Sera from Women with and without Preeclampsia by Mass Spectrometry.
FFPE
HSP90
PRM
S100A6
immunofluorescence
placenta
preeclampsia
serum
Journal
Proteomics. Clinical applications
ISSN: 1862-8354
Titre abrégé: Proteomics Clin Appl
Pays: Germany
ID NLM: 101298608
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
16
05
2018
revised:
11
10
2018
pubmed:
13
11
2018
medline:
31
1
2020
entrez:
13
11
2018
Statut:
ppublish
Résumé
The objective of present study is to determine serum levels and placental distribution of two interacting proteins calcyclin and heat shock protein 90 in preeclampsia. Maternal serum levels of calcyclin and heat shock protein 90 are compared throughout pregnancy from the first trimester till term among women with preeclampsia (n = 43) and age-matched normotensive pregnant controls (n = 46). A serum-based 2D LC-MS assay using Parallel Reaction Monitoring is applied to quantify both calcyclin and heat shock protein 90. Serum levels of calcyclin are significantly lower in patients with preeclampsia in the second trimester of pregnancy as compared to controls (p < 0.05). Serum levels of heat shock protein 90 are significantly higher in patients with preeclampsia in the third trimester as compared to controls (p < 0.001). Both interacting proteins calcyclin and heat shock protein 90 are notably changed in preeclamptic patients compared to controls. Calcyclin is already decreased before the onset of preeclampsia in the second trimester and HSP90 is strongly increased in the third trimester. This suggests that these proteins may play a role in the pathogenesis of preeclampsia and ought to be investigated in large cohort studies as molecular biomarkers.
Identifiants
pubmed: 30417587
doi: 10.1002/prca.201800181
pmc: PMC6588016
doi:
Substances chimiques
HSP90 Heat-Shock Proteins
0
S100 Calcium Binding Protein A6
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1800181Informations de copyright
© 2018 The Authors. Proteomics - Clinical Application Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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