Polymeric ionically conductive composite matrices and electrical stimulation strategies for nerve regeneration: In vitro characterization.

Antigens, Differentiation / metabolism Electric Conductivity Electric Stimulation Therapy Extracellular Matrix / chemistry Humans Mesenchymal Stem Cells / metabolism Nerve Regeneration Neurites / metabolism Polymers / chemistry
electrical stimulation ionic conductivity micro-nanofiber nerve regeneration scaffold

Journal

Journal of biomedical materials research. Part B, Applied biomaterials
ISSN: 1552-4981
Titre abrégé: J Biomed Mater Res B Appl Biomater
Pays: United States
ID NLM: 101234238

Informations de publication

Date de publication:
08 2019
Historique:
received: 05 03 2018
revised: 18 07 2018
accepted: 21 07 2018
pubmed: 13 11 2018
medline: 4 9 2020
entrez: 13 11 2018
Statut: ppublish

Résumé

Stem cell strategies and the use of electrical stimulation (ES) represent promising new frontiers for peripheral nerve regeneration. Composite matrices were fabricated by coating electrospun polycaprolactone/cellulose acetate micro-nanofibers with chitosan and ionically conductive (IC) polymers including, sulfonated polyaniline, and lignin sulfonate. These composite matrices were characterized for surface morphology, coating uniformity, ionic conductivity, and mechanical strength to explore as scaffold materials for nerve regeneration in conjunction with ES. Composite matrices measured conductivity in the range of 0.0049-0.0068 mS/m due to the uniform coating of sulfonated polymers on the micro-nanofibers. Thin films (2D) and composite fiber matrices (3D) of IC polymers seeded with human mesenchymal stem cells (hMSCs) were electrically stimulated at 0.5 V, 20 Hz for 1 h daily for 14 days to study the changes in cell viability, morphology, and expression of the neuronal-like phenotype. In vitro ES lead to changes in hMSCs' fibroblast morphology into elongated neurite-like structures with cell bodies for ES-treated and positive control growth factor-treated groups. Immunofluorescent staining revealed the presence of neuronal markers including β3-tubulin, microtubule-associated protein 2, and nestin in response to ES. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1792-1805, 2019.

Identifiants

DOI: 10.1002/jbm.b.34272 PMID: 30419159 PMC: PMC6511498
pubmed: 30419159
doi: 10.1002/jbm.b.34272
pmc: PMC6511498
mid: NIHMS993187
doi:

Substances chimiques

Antigens, Differentiation 0
Polymers 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

1792-1805

Subventions

Organisme : Connecticut Regenerative Medicine Research Fund
ID : 15-RMBUCHC-08
Pays : International
Organisme : National Science Foundation
ID : EFRI-1332329
Pays : International
Organisme : Division of Graduate Education
ID : DGE-1747453
Pays : International
Organisme : NIBIB NIH HHS
ID : R01EB020640
Pays : United States
Organisme : NIBIB NIH HHS
ID : R01 EB020640
Pays : United States
Organisme : U.S. Department of Defense
ID : OR120140
Pays : International

Informations de copyright

© 2018 Wiley Periodicals, Inc.

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Auteurs

Ohan S Manoukian (OS)

Department of Biomedical Engineering, University of Connecticut, Storrs, Connecticut.
Department of Orthopedic Surgery, University of Connecticut Health, Farmington, Connecticut.

Scott Stratton (S)

Department of Biomedical Engineering, University of Connecticut, Storrs, Connecticut.
Department of Orthopedic Surgery, University of Connecticut Health, Farmington, Connecticut.

Michael R Arul (MR)

Department of Orthopedic Surgery, University of Connecticut Health, Farmington, Connecticut.

Joshua Moskow (J)

Department of Biomedical Engineering, University of Connecticut, Storrs, Connecticut.
Department of Orthopedic Surgery, University of Connecticut Health, Farmington, Connecticut.

Naseem Sardashti (N)

Department of Biomedical Engineering, University of Connecticut, Storrs, Connecticut.

Xiaojun Yu (X)

Department of Chemistry, Chemical Biology and Biomedical Engineering, Stevens Institute of Technology, Hoboken, New Jersey.

Swetha Rudraiah (S)

Department of Orthopedic Surgery, University of Connecticut Health, Farmington, Connecticut.
Department of Pharmaceutical Sciences, University of Saint Joseph, Hartford, Connecticut.

Sangamesh G Kumbar (SG)

Department of Biomedical Engineering, University of Connecticut, Storrs, Connecticut.
Department of Orthopedic Surgery, University of Connecticut Health, Farmington, Connecticut.

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Classifications MeSH

questionsmedicales.fr › Facteurs biologiques › Marqueurs biologiques › Antigènes de différenciation › Polymeric ionically conductive composite...
questionsmedicales.fr › Phénomènes physiques › Phénomènes magnétiques › Phénomènes électromagnétiques › Électricité › Conductivité électrique › Polymeric ionically conductive composite...
questionsmedicales.fr › Thérapeutique › Techniques de physiothérapie › Électrothérapie › Polymeric ionically conductive composite...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace extracellulaire › Matrice extracellulaire › Polymeric ionically conductive composite...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Polymeric ionically conductive composite...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches multipotentes › Cellules souches mésenchymateuses › Polymeric ionically conductive composite...
questionsmedicales.fr › Phénomènes biologiques › Régénération › Régénération nerveuse › Polymeric ionically conductive composite...
questionsmedicales.fr › Cellules › Neurones › Neurites › Polymeric ionically conductive composite...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Polymeric ionically conductive composite...