Preparation of an injectable modified chitosan-based hydrogel approaching for bone tissue engineering.


Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
15 Feb 2019
Historique:
received: 22 07 2018
revised: 13 10 2018
accepted: 08 11 2018
pubmed: 14 11 2018
medline: 11 4 2019
entrez: 14 11 2018
Statut: ppublish

Résumé

This research aimed to design an injectable chitosan-based hydrogel for bone tissue engineering. To this end, chitosan should not only be soluble in water but also be thixotropic. Therefore, it can be used as an injectable liquid that can form a hydrogel under physiological conditions. Accordingly, chitosan was modified with carboxymethyl chloride and the resultant carboxymethyl chitosan (CMCTS) was conjugated to α-cyclodextrin (α-CD) to yield CMCTSCD. The α-CD conjugation improved the water solubility and the hydrophobic cavities to function as crosslinking points by forming an inclusion complex with poly(ethylene glycol) (PEG). The optimum mole ratio of α-CD (as CMCTSCD) to PEG was found to be 2 to 4 with a threading number of ~2.5. The sol-to-gel transition was formed within 450 ± 10 min. The interconnecting porous structure of the gel was observed by scanning electron microscope. Therefore, CMCTSCD/PEG is a potential novel design that can be used as an injectable hydrogel for bone tissue engineering.

Identifiants

pubmed: 30423397
pii: S0141-8130(18)33765-6
doi: 10.1016/j.ijbiomac.2018.11.041
pii:
doi:

Substances chimiques

Biocompatible Materials 0
Hydrogels 0
alpha-Cyclodextrins 0
Polyethylene Glycols 3WJQ0SDW1A
Chitosan 9012-76-4
alpha-cyclodextrin Z1LH97KTRM

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

167-173

Informations de copyright

Copyright © 2018 Elsevier B.V. All rights reserved.

Auteurs

Kanyarat Saekhor (K)

Department of Materials Science, Faculty of Science, Chulalongkorn University, Phayathai, Pathumwan, Bangkok 10330, Thailand.

Wanvisa Udomsinprasert (W)

Department of Biochemistry, Faculty of Pharmacy, Mahidol University, 447 Sri-ayuthaya Road, Rajathevee, Bangkok 10400, Thailand.

Sittisak Honsawek (S)

Department of Biochemistry, Osteoarthritis and Musculoskeleton Research Unit, Faculty of Medicine, Chulalongkorn University, Phayathai, Pathumwan, Bangkok 10330, Thailand.

Wanpen Tachaboonyakiat (W)

Department of Materials Science, Faculty of Science, Chulalongkorn University, Phayathai, Pathumwan, Bangkok 10330, Thailand. Electronic address: Wanpen.Ta@chula.ac.th.

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Classifications MeSH