Evaluation of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for identifying Burkholderia pseudomallei and Burkholderia thailandensis isolates.
16S rRNA gene sequencing analysis
Burkholderia pseudomallei
Burkholderia thailandensis
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
SuperSpectra
Journal
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
ISSN: 1435-4373
Titre abrégé: Eur J Clin Microbiol Infect Dis
Pays: Germany
ID NLM: 8804297
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
20
07
2018
accepted:
29
10
2018
pubmed:
15
11
2018
medline:
22
3
2019
entrez:
15
11
2018
Statut:
ppublish
Résumé
Since Burkholderia thailandensis is included in the reference spectra of the VITEK MS libraries rather than Burkholderia pseudomallei, B. pseudomallei cannot be correctly identified in the current version of VITEK MS. This study was undertaken to evaluate the utility of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) with the VITEK MS plus system in the detection of B. pseudomallei and B. thailandensis isolates. For each species, we increased the reference spectra, and then, a SuperSpectrum was created based on the selection of 39 specific masses. In a second step, we validated the SuperSpectra with 106 isolates identified by 16S rRNA gene sequencing. The results showed that there was 100% agreement between the validation strains analyzed by MALDI-TOF MS and those evaluated using 16S rRNA gene sequencing analysis methods. Therefore, MALDI-TOF MS is a promising, rapid, and economical method to monitor the outbreaks and spread of B. pseudomallei and B. thailandensis isolates.
Identifiants
pubmed: 30426332
doi: 10.1007/s10096-018-3415-3
pii: 10.1007/s10096-018-3415-3
doi:
Substances chimiques
DNA, Bacterial
0
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
191-196Subventions
Organisme : the Special Project of the "Twelfth Five-year Plan" for Medical Science Development of the PLA (AWS14C003-2)
ID : AWS14C003-2
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