Inhibitory effects of Clematis orientalis aqueous ethanol extract and fractions on inflammatory markers in complete Freund's adjuvant-induced arthritis in Sprague-Dawley rats.
Animals
Anti-Inflammatory Agents
/ pharmacology
Antioxidants
/ pharmacology
Antirheumatic Agents
/ pharmacology
Arthritis, Experimental
/ chemically induced
Arthritis, Rheumatoid
/ chemically induced
Biomarkers
/ metabolism
Clematis
/ chemistry
Cytokines
/ metabolism
Ethanol
/ chemistry
Female
Flavonoids
/ pharmacology
Freund's Adjuvant
/ pharmacology
Male
Phytotherapy
Plant Extracts
/ pharmacology
Rats
Rats, Sprague-Dawley
Clematis orientalis
Complete freunds adjuvant
Enzyme-linked immunosorbent assay
Real-time polymerase chain reaction
Rheumatoid arthritis
Journal
Inflammopharmacology
ISSN: 1568-5608
Titre abrégé: Inflammopharmacology
Pays: Switzerland
ID NLM: 9112626
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
received:
01
10
2018
accepted:
29
10
2018
pubmed:
18
11
2018
medline:
10
1
2020
entrez:
18
11
2018
Statut:
ppublish
Résumé
Clematis orientalis Linn has long been used as ethnopharmacy for the treatment of arthritis. This study is intended to evaluate the curative efficacy of Clematis orientalis in treating polyarthritis in rats. Aqueous ethanolic extract and fractions (hexane, butanol and aqueous) were administered orally at 200 mg/kg for 28 days after CFA immunization. Paw swelling, paw diameter, arthritic score, body weight, hematological parameters, radiographic and histological analysis of ankle joints were evaluated. Moreover, levels of various inflammatory markers through RT-PCR and ELISA were measured. DPPH and reducing power assays were used to appraise antioxidant capacity. Qualitative phytochemical analysis, determination of total phenolic and flavonoid contents were also carried out. Aqueous ethanolic extract and fractions significantly (p < 0.001) reduced paw volume, paw thickness and arthritic score and considerably prevented decrease in body weight along with anomalous alterations in hematological parameters in comparison with arthritic control. X-ray and histological examination revealed no significant structural changes in ankle joints of treated rats. Expression levels of IL-1β, TNF-α, IL-6, COX-2 and NF-Kβ were significantly (p < 0.05-0.001) suppressed as well as noteworthy increase in the levels of IL-4 and IL-10 among treated animals has been detected. Overproduction of TNF-α and PGE2 was substantially prevented in animals given different treatments. Aqueous ethanol extract and its fractions demonstrated significant and concentration-dependent antioxidant potential. In general, among fractions aqueous fraction exhibited a greater anti-arthritic effect. Phytochemical analysis of aqueous fraction confirmed the presence of flavonoids and glycosides, 215.29 mgGAE/ml phenolic content and 633.03 μgQE/ml flavonoid content. Thus, we suggest Clematis orientalis as a potent strategy for the treatment of rheumatoid arthritis.
Identifiants
pubmed: 30446927
doi: 10.1007/s10787-018-0543-4
pii: 10.1007/s10787-018-0543-4
doi:
Substances chimiques
Anti-Inflammatory Agents
0
Antioxidants
0
Antirheumatic Agents
0
Biomarkers
0
Cytokines
0
Flavonoids
0
Plant Extracts
0
Ethanol
3K9958V90M
Freund's Adjuvant
9007-81-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
781-797Références
Lancet. 1989 May 20;1(8647):1122-6
pubmed: 2566060
Am J Clin Nutr. 2004 May;79(5):727-47
pubmed: 15113710
Best Pract Res Clin Rheumatol. 2001 Dec;15(5):677-91
pubmed: 11812015
Free Radic Biol Med. 1990;9(1):19-21
pubmed: 2170243
Lancet. 2007 Dec 1;370(9602):1861-74
pubmed: 17570481
Agents Actions. 1988 Aug;25(1-2):94-105
pubmed: 3142230
Lancet. 2010 Sep 25;376(9746):1094-108
pubmed: 20870100
J Ethnopharmacol. 2013 May 20;147(2):327-34
pubmed: 23538163
Int Immunopharmacol. 2012 Jan;12(1):205-11
pubmed: 22155102
Best Pract Res Clin Rheumatol. 2008 Apr;22(2):221-38
pubmed: 18455681
Pharm Biol. 2017 Dec;55(1):792-798
pubmed: 28103731
Mol Cell Biochem. 2014 Sep;394(1-2):67-75
pubmed: 24846408
Arthritis Res. 2002;4(6):333-6
pubmed: 12453308
Arthritis Res. 2001;3(4):200-6
pubmed: 11438035
J Ethnopharmacol. 2017 Jun 9;205:1-7
pubmed: 28457902
BMC Complement Altern Med. 2017 Jul 18;17(1):371
pubmed: 28720131
Biomed Res Int. 2013;2013:635143
pubmed: 23971042
Int Immunopharmacol. 2011 Dec;11(12):2150-8
pubmed: 21983642
Pharm Biol. 2013 Jun;51(6):783-9
pubmed: 23675837
Arthritis. 2012;2012:239310
pubmed: 23193470
Curr Opin Clin Nutr Metab Care. 2008 Nov;11(6):733-40
pubmed: 18827577
J Chin Med Assoc. 2014 Jun;77(6):302-7
pubmed: 24613372
Prev Nutr Food Sci. 2016 Jun;21(2):79-84
pubmed: 27390722
Br Med Bull. 2010;94:201-14
pubmed: 20200013
Nat Rev Drug Discov. 2003 Jun;2(6):473-88
pubmed: 12776222
Planta Med. 1992 Dec;58(6):499-504
pubmed: 1336604
Pharm Biol. 2013 Jan;51(1):13-22
pubmed: 22994412
Nutrients. 2015 Dec 25;8(1):
pubmed: 26712785
J Ethnopharmacol. 2015 Dec 24;176:177-206
pubmed: 26471289
Cold Spring Harb Protoc. 2010 Jun;2010(6):pdb.prot5439
pubmed: 20516177
Br J Pharmacol. 2009 Dec;158(7):1835-47
pubmed: 19888960
Arthritis Rheum. 1988 Mar;31(3):315-24
pubmed: 3358796
Z Rheumatol. 2007 Nov;66(7):591-2, 594
pubmed: 17924123
Lancet. 2009 Feb 21;373(9664):659-72
pubmed: 19157532
Eur J Pharmacol. 2014 Sep 5;738:263-72
pubmed: 24943733
Nutrients. 2016 Mar 15;8(3):167
pubmed: 26999194
Arthritis Rheum. 2005 Nov;52(11):3381-90
pubmed: 16258899
Respir Med. 2002 Aug;96(8):594-8
pubmed: 12195840
Oxid Med Cell Longev. 2011;2011:976701
pubmed: 21912723
J Exp Med. 1988 Mar 1;167(3):1211-27
pubmed: 3351436