Alkaline extracellular conditions promote the proliferation and mineralization of a human cementoblast cell line.
Proliferation
TRPA1
alkaline conditions
calcium
cementoblast
mineralization
Journal
International endodontic journal
ISSN: 1365-2591
Titre abrégé: Int Endod J
Pays: England
ID NLM: 8004996
Informations de publication
Date de publication:
May 2019
May 2019
Historique:
received:
28
05
2018
accepted:
13
11
2018
pubmed:
18
11
2018
medline:
30
10
2019
entrez:
18
11
2018
Statut:
ppublish
Résumé
To investigate the proliferation and mineralization of a human cementoblast cell line under alkaline conditions. A human cementoblast cell line was cultured in alkaline media with several pHs (pH 7.6, 8.0 and 8.4) without CO Cell numbers increased in a time-dependent manner in the high pH medium groups. Western blot analysis revealed the upregulated expression of phospho-p44/42 under alkaline conditions. ALP activity was also increased at pH 8.0 and 8.4. Alizarin red staining revealed increased mineralization in the high pH medium groups. The incorporation of the transient receptor potential ankyrin subfamily member 1 (TRPA1) antagonist HC030031 markedly negated the effect on proliferation and mineralization. Extracellular alkaline conditions promoted the proliferation and mineralization of human cementoblasts in vitro via TRPA1.
Substances chimiques
Alkaline Phosphatase
EC 3.1.3.1
Types de publication
Journal Article
Langues
eng
Pagination
639-645Subventions
Organisme : Multidisciplinary Research Center for Jaw Disease (MRCJD) from Tokyo Dental College
Informations de copyright
© 2018 International Endodontic Journal. Published by John Wiley & Sons Ltd.