Insights into the dynamics of memory, effector and apoptotic cytotoxic T lymphocytes in channel catfish, Ictalurus punctatus.
Effector T cells
Lipid binding
Memory T cells
Natural IgM
Phosphatidylinositol bisphosphate
Teleosts
Journal
Developmental and comparative immunology
ISSN: 1879-0089
Titre abrégé: Dev Comp Immunol
Pays: United States
ID NLM: 7708205
Informations de publication
Date de publication:
03 2019
03 2019
Historique:
received:
30
09
2018
revised:
03
11
2018
accepted:
03
11
2018
pubmed:
18
11
2018
medline:
23
7
2019
entrez:
18
11
2018
Statut:
ppublish
Résumé
In this study, we used the channel catfish model clonal TS32.15 alloantigen-specific cytotoxic T cell (CTL) line to examine the dynamics of memory CTL expansion and senescence in teleosts. Although TS32.15 has been routinely cultured to study catfish CTL responses and killing mechanisms, little is known about the dynamics of the CTLs in these cultures. Here we show that this cell line consists of small non-cytotoxic T cells and larger granular effector T cells and that their ratios vary with time after stimulation. Small CTLs, when exposed to their irradiated targets, replicate and differentiate to morphologically distinct cytotoxic effectors, which do not replicate. After lysing target cells, or with prolonged absence of stimulation, the effector cells transition to a non-cytolytic senescent stage or become apoptotic. In addition, we demonstrate that natural IgM in catfish serum binds lipids, including PIP
Identifiants
pubmed: 30447233
pii: S0145-305X(18)30493-2
doi: 10.1016/j.dci.2018.11.001
pii:
doi:
Substances chimiques
Immunoglobulin M
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
116-128Informations de copyright
Copyright © 2018 Elsevier Ltd. All rights reserved.