The Penaeus stylirostris densovirus capsid interacts with Litopenaeus vannamei troponin I.

Animals Capsid Proteins / metabolism Densovirus / physiology Host-Pathogen Interactions Penaeidae / metabolism Troponin I / metabolism
Capsid Interaction Litopenaeus vannamei Penaeus stylirostris densovirus Troponin I

Journal

Fish & shellfish immunology
ISSN: 1095-9947
Titre abrégé: Fish Shellfish Immunol
Pays: England
ID NLM: 9505220

Informations de publication

Date de publication:
Mar 2019
Historique:
received: 22 08 2018
revised: 15 10 2018
accepted: 14 11 2018
pubmed: 18 11 2018
medline: 6 5 2019
entrez: 18 11 2018
Statut: ppublish

Résumé

The Penaeus stylirostris densovirus (PstDNV) (also known as infectious hypodermal and hematopoietic necrosis virus, IHHNV), a very small DNA virus, is a major shrimp pathogen. The PstDNV genome encodes only two nonstructural proteins and one capsid protein. This virus is thus an ideal, simple model for the investigation of virus-host interactions. To explore the role of the PstDNV capsid in viral infections, a yeast two-hybrid (Y2H) cDNA library was constructed based on Pacific white shrimp, Litopenaeus vannamei mRNA. The Y2H library was then screened, using the PstDNV capsid protein as bait. We identified a host protein that interacted strongly with the PstDNV capsid as L. vannamei troponin I (LvTnI). An in vitro co-immunoprecipitation experiment further supported this interaction. In addition, an in vivo neutralization experiment showed that the vaccination with anti-LvTnI significantly reduced PstDNV copies in PstDNV-challenged shrimp, indicating that the interaction between the PstDNV capsid and cellular LvTnI is essential for PstDNV infection. This result has important implications for our understanding of the mechanisms by which PstDNV infects shrimp.

Identifiants

DOI: 10.1016/j.fsi.2018.11.034 PMID: 30447431
pubmed: 30447431
pii: S1050-4648(18)30753-8
doi: 10.1016/j.fsi.2018.11.034
pii:
doi:

Substances chimiques

Capsid Proteins 0
Troponin I 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

101-106

Informations de copyright

Copyright © 2018 Elsevier Ltd. All rights reserved.

Auteurs

Xiuli Chen (X)

Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fisheries Sciences, Nanning, China.

Digang Zeng (D)

Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fisheries Sciences, Nanning, China.

Weilin Zhu (W)

Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fisheries Sciences, Nanning, China.

Min Peng (M)

Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fisheries Sciences, Nanning, China.

Chunling Yang (C)

Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fisheries Sciences, Nanning, China.

Qiangyong Li (Q)

Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fisheries Sciences, Nanning, China.

Qingyun Liu (Q)

Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fisheries Sciences, Nanning, China.

Qiong Yang (Q)

Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fisheries Sciences, Nanning, China.

Hui Wang (H)

Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fisheries Sciences, Nanning, China.

Min Li (M)

Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fisheries Sciences, Nanning, China.

Yong Lin (Y)

Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fisheries Sciences, Nanning, China.

Yongzhen Zhao (Y)

Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fisheries Sciences, Nanning, China.

Xiaohan Chen (X)

Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fisheries Sciences, Nanning, China. Electronic address: chnxhn@163.com.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › The Penaeus stylirostris densovirus capsid...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales structurales › Protéines nucléocapside › Protéines de capside › The Penaeus stylirostris densovirus capsid...
questionsmedicales.fr › Virus › Virus des insectes › Densovirinae › Densovirus › The Penaeus stylirostris densovirus capsid...
questionsmedicales.fr › Phénomènes biologiques › Interactions hôte-microbes › Interactions hôte-pathogène › The Penaeus stylirostris densovirus capsid...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Crustacea › Decapoda (crustacea) › Penaeidae › The Penaeus stylirostris densovirus capsid...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du cytosquelette › Protéines des microfilaments › Troponine › Troponine I › The Penaeus stylirostris densovirus capsid...