Serological and molecular analysis for brucellosis in selected swine herds from Southern India.
Animals
Antibodies, Bacterial
/ blood
Bacteriological Techniques
Brucella
/ genetics
Brucellosis
/ epidemiology
Disease Transmission, Infectious
Enzyme-Linked Immunosorbent Assay
Female
Humans
India
/ epidemiology
Male
Molecular Diagnostic Techniques
Occupational Exposure
Polymerase Chain Reaction
Pregnancy
Serologic Tests
Swine
Swine Diseases
/ epidemiology
Brucellosis
Isolation
PCR
RBPT
Serology
Swine
iELISA
Journal
Journal of infection and public health
ISSN: 1876-035X
Titre abrégé: J Infect Public Health
Pays: England
ID NLM: 101487384
Informations de publication
Date de publication:
Historique:
received:
25
11
2017
revised:
15
09
2018
accepted:
29
10
2018
pubmed:
19
11
2018
medline:
16
4
2019
entrez:
19
11
2018
Statut:
ppublish
Résumé
Swine brucellosis is a zoonotic disease caused by Brucella suis. The study describes the occurrence of brucellosis in two organized piggeries in Southern India. A total of 585 serum samples comprising 575 from pigs and 10 from animal handlers were collected and tested by serological tests and PCR. Tissue samples were collected for isolation of the pathogen. Out of 575 serum samples screened, 236 (41.04%) were positive for brucellosis by both Rose Bengal plate test (RBPT) and indirect ELISA (iELISA) and 47 (8.17) samples showed Brucella DNA amplification by genus specific PCR. The sows those aborted and 19 boars with orchitis were seropositive for brucellosis indicating association of clinical symptoms with brucellosis seropositivity. Two of 10 pig handlers were positive by RBPT and showed significant serum agglutination test (SAT) titres of >1:160 and 1:320. B. suis bvI was isolated and identified by biochemical tests and confirmed by amplification Brucella genus and Bruce ladder PCRs from vaginal and testicular samples. The introduction of untested breeding boars in the farms might have resulted in the disease transmission and spread. The present study highlighted the diagnosis of B. suis bvI as a cause of abortions in the pigs and occupational exposure to farm personnel.
Sections du résumé
BACKGROUND
BACKGROUND
Swine brucellosis is a zoonotic disease caused by Brucella suis. The study describes the occurrence of brucellosis in two organized piggeries in Southern India.
METHODS
METHODS
A total of 585 serum samples comprising 575 from pigs and 10 from animal handlers were collected and tested by serological tests and PCR. Tissue samples were collected for isolation of the pathogen.
RESULTS
RESULTS
Out of 575 serum samples screened, 236 (41.04%) were positive for brucellosis by both Rose Bengal plate test (RBPT) and indirect ELISA (iELISA) and 47 (8.17) samples showed Brucella DNA amplification by genus specific PCR. The sows those aborted and 19 boars with orchitis were seropositive for brucellosis indicating association of clinical symptoms with brucellosis seropositivity. Two of 10 pig handlers were positive by RBPT and showed significant serum agglutination test (SAT) titres of >1:160 and 1:320. B. suis bvI was isolated and identified by biochemical tests and confirmed by amplification Brucella genus and Bruce ladder PCRs from vaginal and testicular samples.
CONCLUSIONS
CONCLUSIONS
The introduction of untested breeding boars in the farms might have resulted in the disease transmission and spread. The present study highlighted the diagnosis of B. suis bvI as a cause of abortions in the pigs and occupational exposure to farm personnel.
Identifiants
pubmed: 30448370
pii: S1876-0341(18)30183-7
doi: 10.1016/j.jiph.2018.10.013
pii:
doi:
Substances chimiques
Antibodies, Bacterial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
247-251Informations de copyright
Copyright © 2018. Published by Elsevier Ltd.