Coalesced thermal and electrotransfer mediated delivery of plasmid DNA to the skin.
Gene electrotransfer
Gene therapy
Guinea pig model
Heating
Skin
Journal
Bioelectrochemistry (Amsterdam, Netherlands)
ISSN: 1878-562X
Titre abrégé: Bioelectrochemistry
Pays: Netherlands
ID NLM: 100953583
Informations de publication
Date de publication:
Feb 2019
Feb 2019
Historique:
received:
27
11
2017
revised:
21
10
2018
accepted:
21
10
2018
entrez:
20
11
2018
pubmed:
20
11
2018
medline:
20
12
2018
Statut:
ppublish
Résumé
Efficient gene delivery and expression in the skin can be a promising minimally invasive technique for therapeutic clinical applications for immunotherapy, vaccinations, wound healing, cancer, and peripheral artery disease. One of the challenges for efficient gene electrotransfer (GET) to skin in vivo is confinement of expression to the epithelium. Another challenge involves tissue damage. Optimizing gene expression profiles, while minimizing tissue damage are necessary for therapeutic applications. Previously, we established that heating pretreatment to 43 °C enhances GET in vitro. We observed a similar trend in vivo, with an IR-pretreatment for skin heating prior to GET. Currently, we tested a range of GET conditions in vivo in guinea pigs with and without preheating the skin to ~43 °C. IR-laser heating and conduction heating were tested in conjunction with GET. In vivo electrotransfer to the skin by moderately elevating tissue temperature can lead to enhanced gene expression, as well as achieve gene transfer in epidermal, dermal, hypodermal and muscle tissue layers.
Identifiants
pubmed: 30449324
pii: S1567-5394(17)30596-0
doi: 10.1016/j.bioelechem.2018.10.004
pmc: PMC7328822
mid: NIHMS1597672
pii:
doi:
Substances chimiques
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
127-133Subventions
Organisme : NIBIB NIH HHS
ID : R01 EB018956
Pays : United States
Informations de copyright
Copyright © 2018 Elsevier B.V. All rights reserved.
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