Terminal Deoxynucleotidyl Transferase in the Synthesis and Modification of Nucleic Acids.
DNA
end labeling
immobilization
nucleotides
terminal deoxynucleotidyl transferase
Journal
Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360
Informations de publication
Date de publication:
01 04 2019
01 04 2019
Historique:
received:
27
10
2018
pubmed:
20
11
2018
medline:
8
1
2020
entrez:
20
11
2018
Statut:
ppublish
Résumé
The terminal deoxynucleotidyl transferase (TdT) belongs to the X family of DNA polymerases. This unusual polymerase catalyzes the template-independent addition of random nucleotides on 3'-overhangs during V(D)J recombination. The biological function and intrinsic biochemical properties of the TdT have spurred the development of numerous oligonucleotide-based tools and methods, especially if combined with modified nucleoside triphosphates. Herein, we summarize the different applications stemming from the incorporation of modified nucleotides by the TdT. The structural, mechanistic, and biochemical properties of this polymerase are also discussed.
Identifiants
pubmed: 30451377
doi: 10.1002/cbic.201800658
doi:
Substances chimiques
DNA, Single-Stranded
0
Metals
0
Nucleotides
0
Oligonucleotides
0
DNA Nucleotidylexotransferase
EC 2.7.7.31
DNA-Directed DNA Polymerase
EC 2.7.7.7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
860-871Informations de copyright
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.