Electron tomography in plant cell biology.
Arabidopsis
/ ultrastructure
Chlamydomonas reinhardtii
/ ultrastructure
Cryoelectron Microscopy
/ methods
Electron Microscope Tomography
/ methods
Image Processing, Computer-Assisted
/ methods
Imaging, Three-Dimensional
/ methods
Microscopy, Electron, Transmission
/ methods
Plant Cells
/ ultrastructure
Plant Structures
/ ultrastructure
cryo-electron microscopy
electron tomography
plant biology
Journal
Microscopy (Oxford, England)
ISSN: 2050-5701
Titre abrégé: Microscopy (Oxf)
Pays: England
ID NLM: 101595834
Informations de publication
Date de publication:
01 Feb 2019
01 Feb 2019
Historique:
received:
30
08
2018
revised:
15
10
2018
accepted:
31
10
2018
pubmed:
20
11
2018
medline:
23
8
2019
entrez:
20
11
2018
Statut:
ppublish
Résumé
Electron tomography (ET) approaches are based on the imaging of a biological specimen at different tilt angles by transmission electron microscopy (TEM). ET can be applied to both plastic-embedded and frozen samples. Technological advancements in TEM, direct electron detection, automated image collection, and imaging processing algorithms allow for 2-7-nm scale axial resolution in tomographic reconstructions of cells and organelles. In this review, we discussed the application of ET in plant cell biology and new opportunities for imaging plant cells by cryo-ET and other 3D electron microscopy approaches.
Identifiants
pubmed: 30452668
pii: 5185954
doi: 10.1093/jmicro/dfy133
doi:
Types de publication
Journal Article
Review
Langues
eng
Pagination
69-79Informations de copyright
© The Author(s) 2018. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.