Class A scavenger receptors mediate extracellular dsRNA sensing, leading to downstream antiviral gene expression in a novel American toad cell line, BufoTad.
Amphibian Proteins
/ genetics
Animals
Antiviral Agents
/ metabolism
Anura
/ physiology
Cell Line
Extracellular Space
Immunity, Innate
Ligands
Pathogen-Associated Molecular Pattern Molecules
/ immunology
Protein Binding
RNA, Double-Stranded
/ genetics
RNA, Viral
/ genetics
Scavenger Receptors, Class A
/ genetics
Signal Transduction
AcLDL
American toad
Amphibian
Class A scavenger receptor
Mx
dsRNA
Journal
Developmental and comparative immunology
ISSN: 1879-0089
Titre abrégé: Dev Comp Immunol
Pays: United States
ID NLM: 7708205
Informations de publication
Date de publication:
03 2019
03 2019
Historique:
received:
05
10
2018
revised:
15
11
2018
accepted:
15
11
2018
pubmed:
20
11
2018
medline:
23
7
2019
entrez:
20
11
2018
Statut:
ppublish
Résumé
Viral double-stranded (ds)RNA is a potent pathogen-associated molecular pattern (PAMP), capable of inducing a strong antiviral state within the cell, protecting the cell from virus infection. In mammals and fish, sensing extracellular dsRNA is mediated by cell-surface class A scavenger receptors (SR-As). Currently, very little is known about SR-As in amphibians, including: sequence, expression patterns and function. To this end, SR-A expression and function was studied in a novel American toad (Anaxyrus americanus) tadpole cell line called BufoTad. BufoTad was derived from a whole tadpole. The cell line exhibits a cobblestone morphology and expresses abundant levels of transcripts for cytokeratin 19, vimentin, claudin 3, chemokine receptor CXCR4, and SR-AI, one of the five members of the SR-A family, collectively suggesting that BufoTad could be endothelial-like. BufoTad cells bound acetylated LDL, whereas the Xenopus laevis kidney epithelial A6 cell line did not, suggesting functional SR-A activity in BufoTad cells. Additionally, three SR-A competitive ligands (DxSO
Identifiants
pubmed: 30452932
pii: S0145-305X(18)30500-7
doi: 10.1016/j.dci.2018.11.012
pii:
doi:
Substances chimiques
Amphibian Proteins
0
Antiviral Agents
0
Ligands
0
Pathogen-Associated Molecular Pattern Molecules
0
RNA, Double-Stranded
0
RNA, Viral
0
Scavenger Receptors, Class A
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
140-149Informations de copyright
Copyright © 2018 Elsevier Ltd. All rights reserved.