Safety evaluation of β-agarase preparations from Streptomyces coelicolor A3(2).
Agarase
DagA
Safety
Streptomyces coelicolor
Toxicity
Journal
Regulatory toxicology and pharmacology : RTP
ISSN: 1096-0295
Titre abrégé: Regul Toxicol Pharmacol
Pays: Netherlands
ID NLM: 8214983
Informations de publication
Date de publication:
Feb 2019
Feb 2019
Historique:
received:
17
09
2018
revised:
05
11
2018
accepted:
12
11
2018
pubmed:
20
11
2018
medline:
25
1
2019
entrez:
20
11
2018
Statut:
ppublish
Résumé
Recent studies on neoagarooligosaccharides prepared by hydrolyzing agar with β-agarase DagA produced from Streptomyces coelicolor A3(2) have enhanced our knowledge about the enzymatic utility of S. coelicolor. For safety evaluation, a crude extracellular protein containing DagA (crDagA) was prepared from the culture broth of S. coelicolor A3(2) M22-2C43, a highly productive strain of DagA. All genotoxicity tests, such as bacterial reverse mutation assay, eukaryotic chromosomal aberration assay, and in vivo micronucleus assay in mice showed no mutagenic activity of crDagA. No abnormalities were found in the appearance or behavior upon single oral administration up to 20,000 mg/kg body weight (BW) [318 mg TOS (Total Organic Solids)/kg BW] and long-term repeated oral administration toxicity tests up to 10,000 mg/kg BW/day (159 mg TOS/kg BW/day) in Sprague Dawley
Identifiants
pubmed: 30453009
pii: S0273-2300(18)30297-6
doi: 10.1016/j.yrtph.2018.11.005
pii:
doi:
Substances chimiques
Glycoside Hydrolases
EC 3.2.1.-
agarase
EC 3.2.1.81
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
142-155Informations de copyright
Copyright © 2018 Elsevier Inc. All rights reserved.