Posttransplant Characterization of Long-term Functional hESC-Derived Pancreatic Endoderm Grafts.
Journal
Diabetes
ISSN: 1939-327X
Titre abrégé: Diabetes
Pays: United States
ID NLM: 0372763
Informations de publication
Date de publication:
May 2019
May 2019
Historique:
received:
18
07
2018
accepted:
06
11
2018
pubmed:
21
11
2018
medline:
4
7
2019
entrez:
21
11
2018
Statut:
ppublish
Résumé
The paucity of human donors limits broadened application of β-cell replacement therapy. Insulin-producing cells derived from human embryonic stem cells (hESCs) have recently been investigated clinically as a feasible surrogate to primary tissue. Herein, we examine the long-term efficacy of hESC-derived pancreatic endoderm cells (PECs) to maintain normoglycemia posttransplant and characterize the phenotype of the PEC grafts. Mice with chemically induced diabetes were transplanted with PECs into the subcutaneous device-less site. Transplant function was assessed through nonfasting blood glucose measurements, intraperitoneal glucose tolerance testing (IPGTT), and human C-peptide secretion for 517 days. Explanted grafts were assessed for ex vivo function and immunohistochemically. All PEC recipients (
Identifiants
pubmed: 30455375
pii: db18-0788
doi: 10.2337/db18-0788
doi:
Substances chimiques
Blood Glucose
0
C-Peptide
0
Insulin
0
Types de publication
Journal Article
Comment
Langues
eng
Sous-ensembles de citation
IM
Pagination
953-962Commentaires et corrections
Type : CommentIn
Type : CommentOn
Informations de copyright
© 2019 by the American Diabetes Association.