A new derivatization method for the determination of valproic acid in serum using tetramethylammonium hydroxide as a catalyst.
catalyst
derivatization
high-performance liquid chromatography
tetramethylammonium hydroxide
valproic acid
Journal
Biomedical chromatography : BMC
ISSN: 1099-0801
Titre abrégé: Biomed Chromatogr
Pays: England
ID NLM: 8610241
Informations de publication
Date de publication:
Mar 2019
Mar 2019
Historique:
received:
20
07
2018
revised:
03
11
2018
accepted:
13
11
2018
pubmed:
21
11
2018
medline:
5
3
2019
entrez:
21
11
2018
Statut:
ppublish
Résumé
Valproic acid (VPA) pharmacokinetics is highly variable and monitoring of blood levels is necessary to determine its appropriate dosage. This study aimed to establish and validate a novel derivatization method for the determination of VPA. The method was based on the catalytic effect of tetramethylammonium hydroxide using 2,4'-dibromoacetophenone as a derivatization reagent. After derivatization, samples were injected into the HPLC system for analysis. The method showed a good linearity in the range of 1.0-200.7 μg mL
Substances chimiques
Quaternary Ammonium Compounds
0
Valproic Acid
614OI1Z5WI
tetramethylammonium
H0W55235FC
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e4440Subventions
Organisme : Beijing Municipal Administration of Hospitals' Youth Programme
ID : QML20170506
Organisme : Young Scientist Program
ID : YSP201703
Organisme : Beijng Municipal Administration of Hospitals' Sailing Programme
ID : ZYLX201827
Informations de copyright
© 2018 John Wiley & Sons, Ltd.