In vitro gill cell monolayer successfully reproduces in vivo Atlantic salmon host responses to Neoparamoeba perurans infection.
AG-2
AGD
Amoebic gill disease
Neoparamoeba perurans
Phagocytosis
RTgill-W1
Th2
Transwell(®) permeable supports
Journal
Fish & shellfish immunology
ISSN: 1095-9947
Titre abrégé: Fish Shellfish Immunol
Pays: England
ID NLM: 9505220
Informations de publication
Date de publication:
Mar 2019
Mar 2019
Historique:
received:
17
07
2018
revised:
05
11
2018
accepted:
13
11
2018
pubmed:
21
11
2018
medline:
28
8
2019
entrez:
21
11
2018
Statut:
ppublish
Résumé
An in vitro model to study the host response to Neoparamoeba perurans, the causative agent of amoebic gill disease (AGD), was evaluated. The rainbow trout gill derived cell line, RTgill-W1, was seeded onto permeable cell culture supports and maintained asymmetrically with apical seawater. Cells were inoculated with either a passage attenuated or a recent wild clone of N. perurans. Amoebae, loaded with phagocytosed fluorescent beads, were observed associated with host cells within 20 min post inoculation (pi). By 6 h small foci of cytopathic effect appeared and at 72 h cytolysis was observed, with total disruption of the cell monolayer at 96 h pi. Due to cell monolayer disruption, the platform could not support proliferation of amoebae, which showed a 3-log reduction in parasite 18S rRNA mRNA after 72 h (10
Identifiants
pubmed: 30458309
pii: S1050-4648(18)30748-4
doi: 10.1016/j.fsi.2018.11.029
pmc: PMC6380893
pii:
doi:
Substances chimiques
RNA, Ribosomal, 18S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
287-300Informations de copyright
Crown Copyright © 2018. Published by Elsevier Ltd. All rights reserved.
Références
Immunology. 2000 Jan;99(1):153-61
pubmed: 10651954
J Exp Biol. 2000 May;203(Pt 10):1523-37
pubmed: 10769215
Dis Aquat Organ. 2000 Sep 28;42(3):215-9
pubmed: 11104073
Int J Radiat Biol. 2001 Oct;77(10):1007-21
pubmed: 11682006
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Nucleic Acids Res. 2002 May 1;30(9):e36
pubmed: 11972351
Clin Microbiol Rev. 2002 Jul;15(3):342-54
pubmed: 12097243
FEMS Microbiol Lett. 2003 Jun 6;223(1):41-6
pubmed: 12798998
J Fish Dis. 2004 Mar;27(3):151-61
pubmed: 15009241
J Fish Dis. 2004 Aug;27(8):445-9
pubmed: 15291786
J Fish Dis. 2005 Jan;28(1):49-64
pubmed: 15660793
J Fish Dis. 2005 May;28(5):293-9
pubmed: 15892755
Fish Shellfish Immunol. 2005 Nov;19(5):429-39
pubmed: 15916905
Fish Shellfish Immunol. 2006 Mar;20(3):346-64
pubmed: 16006146
Dis Aquat Organ. 2005 Sep 5;66(2):135-44
pubmed: 16231639
Physiol Genomics. 2006 Jun 16;26(1):15-34
pubmed: 16493018
BMC Bioinformatics. 2006 Apr 07;7:194
pubmed: 16600052
J Allergy Clin Immunol. 2006 Jun;117(6):1455-61
pubmed: 16751013
J Fish Dis. 2006 Aug;29(8):467-80
pubmed: 16911534
Vet Immunol Immunopathol. 2006 Nov 15;114(1-2):121-34
pubmed: 16956669
Dis Aquat Organ. 2007 Feb 8;74(1):57-65
pubmed: 17425264
Int J Parasitol. 2007 Nov;37(13):1469-81
pubmed: 17561022
Fish Shellfish Immunol. 2007 Nov;23(5):1015-31
pubmed: 17566761
J Fish Dis. 2008 Mar;31(3):205-14
pubmed: 18261034
Mol Immunol. 2008 May;45(9):2581-97
pubmed: 18282602
J Fish Dis. 2008 Apr;31(4):285-95
pubmed: 18353020
Dis Aquat Organ. 2008 Jan 24;78(3):217-23
pubmed: 18380220
BMC Bioinformatics. 2008 Jun 06;9:267
pubmed: 18534040
Dev Comp Immunol. 2008;32(12):1539-60
pubmed: 18621418
J Fish Dis. 2008 Sep;31(9):699-705
pubmed: 18786032
In Vitro Cell Dev Biol Anim. 2009 Mar-Apr;45(3-4):127-34
pubmed: 19184248
Immunol Cell Biol. 2010 Mar-Apr;88(3):236-9
pubmed: 20157328
J Fish Dis. 2010 May;33(5):455-8
pubmed: 20158576
Nat Rev Immunol. 2010 Apr;10(4):225-35
pubmed: 20336151
Dis Aquat Organ. 2010 Sep 17;91(3):257-62
pubmed: 21133325
Ann Bot. 2011 May;107(7):1127-40
pubmed: 21169293
J Mol Biol. 1990 Oct 5;215(3):403-10
pubmed: 2231712
Fish Shellfish Immunol. 2012 Jun;32(6):1185-90
pubmed: 22433573
Int J Parasitol. 2012 May 1;42(5):511-5
pubmed: 22549025
J Fish Dis. 2012 Nov;35(11):839-48
pubmed: 22882640
Infect Immun. 2012 Dec;80(12):4456-62
pubmed: 23045476
Fish Shellfish Immunol. 2013 Mar;34(3):789-98
pubmed: 23306092
J Trop Med. 2013;2013:890603
pubmed: 23476670
Dev Comp Immunol. 2014 Apr;43(2):257-67
pubmed: 23921258
J Fish Dis. 2014 Sep;37(9):771-83
pubmed: 23952070
Biomicrofluidics. 2013 Oct 29;7(5):51502
pubmed: 24273628
Fish Shellfish Immunol. 2014 Feb;36(2):563-70
pubmed: 24378682
J Exp Biol. 2014 Mar 1;217(Pt 5):639-50
pubmed: 24574380
Fish Shellfish Immunol. 2014 Jun;38(2):294-302
pubmed: 24721287
Eur J Protistol. 2014 Oct;50(5):509-23
pubmed: 25243758
J Lab Autom. 2015 Apr;20(2):107-26
pubmed: 25586998
Int J Parasitol. 2015 Aug;45(9-10):575-8
pubmed: 26008963
Cold Spring Harb Perspect Biol. 2015 Jun 08;7(7):a019216
pubmed: 26054743
Fish Shellfish Immunol. 2015 Oct;46(2):417-25
pubmed: 26166456
Parasitol Res. 1989;76(1):20-5
pubmed: 2622894
J Fish Dis. 2016 Sep;39(9):1113-23
pubmed: 26775899
PLoS One. 2016 Jul 08;11(7):e0158151
pubmed: 27391648
Parasitology. 2017 Aug;144(9):1229-1242
pubmed: 28492111
Fish Shellfish Immunol. 2017 Jul;66:207-216
pubmed: 28501445
Ecotoxicology. 2017 Dec;26(10):1314-1326
pubmed: 29127661
Trends Parasitol. 2018 May;34(5):404-419
pubmed: 29422444
Appl Environ Microbiol. 1980 Apr;39(4):681-5
pubmed: 6769389
Exp Parasitol. 1994 Mar;78(2):230-41
pubmed: 8119377
Vet Rec. 1996 Oct 5;139(14):348-9
pubmed: 8903016
Dis Aquat Organ. 1998 Jun 19;33(2):137-41
pubmed: 9722402