A New Mechanism for High-Affinity Uptake of C4-Dicarboxylates in Bacteria Revealed by the Structure of Rhodopseudomonas palustris MatC (RPA3494), a Periplasmic Binding Protein of the Tripartite Tricarboxylate Transporter (TTT) Family.
Amino Acid Sequence
Bacterial Proteins
/ metabolism
Biological Transport
/ physiology
Carrier Proteins
/ metabolism
Fumarates
/ metabolism
Malates
/ metabolism
Membrane Transport Proteins
/ metabolism
Periplasm
/ metabolism
Periplasmic Binding Proteins
/ metabolism
Rhodopseudomonas
/ metabolism
Succinic Acid
/ metabolism
malate
periplasm
secondary transporter
substrate binding-protein
tryptophan fluorescence
Journal
Journal of molecular biology
ISSN: 1089-8638
Titre abrégé: J Mol Biol
Pays: Netherlands
ID NLM: 2985088R
Informations de publication
Date de publication:
18 01 2019
18 01 2019
Historique:
received:
24
09
2018
revised:
14
11
2018
accepted:
14
11
2018
pubmed:
25
11
2018
medline:
3
3
2020
entrez:
25
11
2018
Statut:
ppublish
Résumé
C4-dicarboxylates play a central role in cellular physiology as key metabolic intermediates. Under aerobic conditions, they participate in the citric acid cycle, while in anaerobic bacteria, they are important in energy-conserving fermentation and respiration processes. Ten different families of secondary transporters have been described to participate in C4-dicarboxylate movement across biological membranes, but only one of these utilizes an extracytoplasmic solute binding protein to achieve high-affinity uptake. Here, we identify the MatBAC system from the photosynthetic bacterium Rhodopseudomonas palustris as the first member of the tripartite tricarboxylate transport family to be involved in C4-dicarboxylate transport. Tryptophan fluorescence spectroscopy showed that MatC, the periplasmic binding protein from this system, binds to l- and d-malate with K
Identifiants
pubmed: 30471256
pii: S0022-2836(18)30977-X
doi: 10.1016/j.jmb.2018.11.016
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Carrier Proteins
0
Fumarates
0
Malates
0
Membrane Transport Proteins
0
Periplasmic Binding Proteins
0
citrate-binding transport protein
0
malic acid
817L1N4CKP
Succinic Acid
AB6MNQ6J6L
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
351-367Informations de copyright
Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.