Measurement of Metabolite Changes in Senescent Cells by Mass Spectrometry.
Mass spectrometry
Metabolites
Metabolomics
Senescence
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
27
11
2018
pubmed:
27
11
2018
medline:
17
8
2019
Statut:
ppublish
Résumé
Senescent cells display altered metabolic signatures that can either be causal for aspects the senescent phenotype, or act as biomarkers for senescence. Steady state levels of multiple metabolites change with senescence, and can be detected using analytical methods. Here, we describe a liquid chromatography-mass spectrometry (LC-MS) method for detecting altered metabolites from cultured senescent cells.
Identifiants
pubmed: 30474846
doi: 10.1007/978-1-4939-8931-7_13
doi:
Substances chimiques
Biomarkers
0
Types de publication
Journal Article
Langues
eng