Binding of a Telomestatin Derivative Changes the Mechanical Anisotropy of a Human Telomeric G-Quadruplex.
G-quadruplexes
induced fit
ligand binding
mechanical anisotropy
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
14 01 2019
14 01 2019
Historique:
received:
25
09
2018
pubmed:
27
11
2018
medline:
29
7
2020
entrez:
27
11
2018
Statut:
ppublish
Résumé
Mechanical anisotropy is an essential property for biomolecules to assume structural and functional roles in mechanobiology. However, there is insufficient information on the mechanical anisotropy of ligand-biomolecule complexes. Herein, we investigated the mechanical property of individual human telomeric G-quadruplexes bound to telomestatin, using optical tweezers. Stacking of the ligand to the G-tetrad planes changes the conformation of the G-quadruplex, which resembles a balloon squeezed in certain directions. Such a squeezed balloon effect strengthens the G-tetrad planes, but dislocates and weakens the loops in the G-quadruplex upon ligand binding. These dynamic interactions indicate that the binding between the ligand and G-quadruplex follows the induced-fit model. We anticipate that the altered mechanical anisotropy of the ligand-G-quadruplex complex can add additional level of regulations on the motor enzymes that process DNA or RNA molecules.
Identifiants
pubmed: 30476359
doi: 10.1002/anie.201811046
pmc: PMC6669079
mid: NIHMS1040868
doi:
Substances chimiques
Oxazoles
0
telomestatin
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
877-881Subventions
Organisme : National Science Foundation
ID : 1415883
Pays : International
Organisme : National Science Foundation
ID : 1609504
Pays : International
Organisme : Japan Society for the Promotion of Science
ID : 26282214
Pays : International
Organisme : Japan Society for the Promotion of Science
ID : 21655060
Pays : International
Organisme : Japan Society for the Promotion of Science
ID : 23310158
Pays : International
Organisme : NCI NIH HHS
ID : R01 CA236350
Pays : United States
Informations de copyright
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Références
Genetica. 1999;106(1-2):15-36
pubmed: 10710707
Nat Struct Biol. 2003 Sep;10(9):738-43
pubmed: 12923571
Nat Struct Biol. 2003 Sep;10(9):731-7
pubmed: 12923573
Proc Natl Acad Sci U S A. 1962 Dec 15;48:2013-8
pubmed: 13947099
Proc Natl Acad Sci U S A. 2006 Aug 22;103(34):12724-8
pubmed: 16908850
Nature. 2007 Apr 12;446(7137):820-3
pubmed: 17361130
Nucleic Acids Res. 2007;35(7):2440-50
pubmed: 17395643
J Mol Biol. 2008 Oct 10;382(3):628-37
pubmed: 18647607
Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15755-60
pubmed: 18852468
Nat Chem. 2011 Aug 28;3(10):782-7
pubmed: 21941250
J Am Chem Soc. 2012 Aug 1;134(30):12338-41
pubmed: 22799529
Biophys J. 2012 Dec 5;103(11):2361-8
pubmed: 23283235
J Am Chem Soc. 2013 Sep 11;135(36):13495-501
pubmed: 23909929
J Am Chem Soc. 2014 Nov 5;136(44):15537-44
pubmed: 25296000
J Am Chem Soc. 2017 Jun 7;139(22):7476-7484
pubmed: 28505453
Angew Chem Int Ed Engl. 2017 Aug 1;56(32):9376-9380
pubmed: 28631866
Science. 1995 Dec 8;270(5242):1653-7
pubmed: 7502073
J Mol Biol. 1996 Dec 6;264(3):534-45
pubmed: 8969303