Non-invasive Vagus Nerve Stimulation Protects Against Cerebral Ischemia/Reperfusion Injury and Promotes Microglial M2 Polarization Via Interleukin-17A Inhibition.
Cerebral ischemia
IL-17A
MCAO
Microglia
Vagus nerve stimulation
Journal
Journal of molecular neuroscience : MN
ISSN: 1559-1166
Titre abrégé: J Mol Neurosci
Pays: United States
ID NLM: 9002991
Informations de publication
Date de publication:
Feb 2019
Feb 2019
Historique:
received:
04
08
2018
accepted:
21
11
2018
pubmed:
30
11
2018
medline:
21
3
2019
entrez:
29
11
2018
Statut:
ppublish
Résumé
Microglia play an essential role during cerebral an ischemia/reperfusion (I/R)-related inflammatory process. Because the M2 phenotype of microglia exhibits anti-inflammation activity, it has become a promising target for anti-inflammatory therapy. Vagus nerve stimulation (VNS) reportedly has neuroprotective effects against cerebral I/R injuries via its anti-inflammatory action. The aim of this study was to investigate the ability of non-invasive VNS (nVNS) to alleviate cerebral I/R in mice by promoting microglial M2 polarization. Neurological scoring and cerebral infarct volume assessments were performed 72 h after a middle cerebral artery occlusion (MCAO)-induced stroke. M2 phenotype microglia were identified by immunohistochemistry staining using Arg-1 and Iba-1 antibodies. The protein expressions of Arg-1, IL-17A, IL-10, Bax, and Bcl-2 were detected by Western blot. Apoptotic cells were detected using TUNEL staining. According to our results, nVNS decreased infarct volume, improved neurological outcomes, reduced apoptotic neurons (TUNEL
Identifiants
pubmed: 30484061
doi: 10.1007/s12031-018-1227-7
pii: 10.1007/s12031-018-1227-7
doi:
Substances chimiques
Interleukin-17
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
217-226Subventions
Organisme : Construction Project of clinical research Center for Chinese Medicine Encephalopathy of Shaanxi Province
ID : 201704
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