Atrial fibrillation and electrophysiology in transgenic mice with cardiac-restricted overexpression of FKBP12.
action potential duration
cardiac electrophysiology
fibrosis
ion channels
patch clamp
voltage-gated sodium current
Journal
American journal of physiology. Heart and circulatory physiology
ISSN: 1522-1539
Titre abrégé: Am J Physiol Heart Circ Physiol
Pays: United States
ID NLM: 100901228
Informations de publication
Date de publication:
01 02 2019
01 02 2019
Historique:
pubmed:
1
12
2018
medline:
28
12
2019
entrez:
1
12
2018
Statut:
ppublish
Résumé
Cardiomyocyte-restricted overexpression of FK506-binding protein 12 transgenic (αMyHC-FKBP12) mice develop spontaneous atrial fibrillation (AF). The aim of the present study is to explore the mechanisms underlying the occurrence of AF in αMyHC-FKBP12 mice. Spontaneous AF was documented by telemetry in vivo and Langendorff-perfused hearts of αMyHC-FKBP12 and littermate control mice in vitro. Atrial conduction velocity was evaluated by optical mapping. The patch-clamp technique was applied to determine the potentially altered electrophysiology in atrial myocytes. Channel protein expression levels were evaluated by Western blot analyses. Spontaneous AF was recorded in four of seven αMyHC-FKBP12 mice but in none of eight nontransgenic (NTG) controls. Atrial conduction velocity was significantly reduced in αMyHC-FKBP12 hearts compared with NTG hearts. Interestingly, the mean action potential duration at 50% but not 90% was significantly prolonged in αMyHC-FKBP12 atrial myocytes compared with their NTG counterparts. Consistent with decreased conduction velocity, average peak Na
Identifiants
pubmed: 30499712
doi: 10.1152/ajpheart.00486.2018
pmc: PMC6397388
doi:
Substances chimiques
CACNA1C protein, mouse
0
Calcium Channels, L-Type
0
NAV1.5 Voltage-Gated Sodium Channel
0
Scn5a protein, mouse
0
Tacrolimus Binding Protein 1A
EC 5.2.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
H371-H379Subventions
Organisme : NHLBI NIH HHS
ID : P01 HL134599
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL145060
Pays : United States
Organisme : NHLBI NIH HHS
ID : R21 HL106554
Pays : United States
Commentaires et corrections
Type : ErratumIn
Références
Pharmacotherapy. 1997 Nov-Dec;17(6):1328-30
pubmed: 9399620
Transplantation. 1998 Aug 27;66(4):535-7
pubmed: 9734501
J Am Coll Cardiol. 2010 May 25;55(21):2330-42
pubmed: 20488304
Circ Res. 1996 Dec;79(6):1110-21
pubmed: 8943949
Circulation. 2008 Apr 15;117(15):1927-35
pubmed: 18378609
Circ Res. 2011 Apr 29;108(9):1042-52
pubmed: 21372286
Circ Cardiovasc Qual Outcomes. 2011 May;4(3):313-20
pubmed: 21540439
Circulation. 2010 Dec 7;122(23):2378-87
pubmed: 21098446
Circulation. 2009 Feb 3;119(4):606-18
pubmed: 19188521
Circulation. 2005 Sep 27;112(13):1927-35
pubmed: 16172272
Circulation. 2005 Apr 12;111(14):1738-46
pubmed: 15809371
Heart Rhythm. 2008 Jan;5(1):99-105
pubmed: 18088563
Circ Res. 2007 Nov 9;101(10):1020-9
pubmed: 17872463
Nature. 1998 Jan 29;391(6666):489-92
pubmed: 9461216
Circulation. 2006 Dec 12;114(24):2584-94
pubmed: 17145985
Physiol Rev. 2004 Apr;84(2):431-88
pubmed: 15044680
J Pharmacol Exp Ther. 2003 Jan;304(1):334-41
pubmed: 12490609
Am J Physiol Cell Physiol. 2010 Dec;299(6):C1234-49
pubmed: 20844252
Circ Arrhythm Electrophysiol. 2008 Apr;1(1):62-73
pubmed: 19808395
Pharmacol Ther. 2001 Nov-Dec;92(2-3):213-30
pubmed: 11916538
Transplantation. 1997 Jul 15;64(1):182-3
pubmed: 9233725
J Mol Cell Cardiol. 2002 Sep;34(9):1185-94
pubmed: 12392892
Cardiovasc Res. 2008 Apr 1;78(1):45-52
pubmed: 18178574
Trends Cardiovasc Med. 2008 Apr;18(3):78-87
pubmed: 18436145
Cell. 1996 Aug 9;86(3):435-44
pubmed: 8756725
Circulation. 2011 Aug 30;124(9):1001-11
pubmed: 21824921
Circulation. 2007 Sep 25;116(13):1449-57
pubmed: 17785620
Science. 1998 Aug 7;281(5378):818-21
pubmed: 9694652
Herz. 2007 May;32(3):185-91
pubmed: 17497250
Eur Heart J. 2006 Apr;27(8):949-53
pubmed: 16527828
Clin Pharmacol Ther. 2007 Jan;81(1):35-41
pubmed: 17185997
J Physiol. 1997 Apr 15;500 ( Pt 2):343-54
pubmed: 9147322
Cardiovasc Res. 2002 May;54(2):405-15
pubmed: 12062345
Cardiovasc Res. 2007 Dec 1;76(3):409-17
pubmed: 17897635
PLoS One. 2012;7(2):e31956
pubmed: 22363773