Developmental origins of transgenerational sperm DNA methylation epimutations following ancestral DDT exposure.
DNA methylation
Epigenetic
Epimutation
Primordial germ cells
Sperm
Spermatogenesis
Testis
Transgenerational
Journal
Developmental biology
ISSN: 1095-564X
Titre abrégé: Dev Biol
Pays: United States
ID NLM: 0372762
Informations de publication
Date de publication:
15 01 2019
15 01 2019
Historique:
received:
05
09
2018
revised:
01
11
2018
accepted:
26
11
2018
pubmed:
1
12
2018
medline:
31
7
2019
entrez:
1
12
2018
Statut:
ppublish
Résumé
Epigenetic alterations in the germline can be triggered by a number of different environmental factors from diet to toxicants. These environmentally induced germline changes can promote the epigenetic transgenerational inheritance of disease and phenotypic variation. In previous studies, the pesticide DDT was shown to promote the transgenerational inheritance of sperm differential DNA methylation regions (DMRs), also called epimutations, which can in part mediate this epigenetic inheritance. In the current study, the developmental origins of the transgenerational DMRs during gametogenesis have been investigated. Male control and DDT lineage F3 generation rats were used to isolate embryonic day 16 (E16) prospermatogonia, postnatal day 10 (P10) spermatogonia, adult pachytene spermatocytes, round spermatids, caput epididymal spermatozoa, and caudal sperm. The DMRs between the control versus DDT lineage samples were determined at each developmental stage. The top 100 statistically significant DMRs at each stage were compared and the developmental origins of the caudal epididymal sperm DMRs were assessed. The chromosomal locations and genomic features of the different stage DMRs were analyzed. Although previous studies have demonstrated alterations in the DMRs of primordial germ cells (PGCs), the majority of the DMRs identified in the caudal sperm originated during the spermatogonia stages in the testis. Interestingly, a cascade of epigenetic alterations initiated in the PGCs is required to alter the epigenetic programming during spermatogenesis to obtain the sperm epigenetics involved in the epigenetic transgenerational inheritance phenomenon.
Identifiants
pubmed: 30500333
pii: S0012-1606(18)30598-0
doi: 10.1016/j.ydbio.2018.11.016
pmc: PMC6338333
mid: NIHMS1516182
pii:
doi:
Substances chimiques
Mutagens
0
Pesticides
0
DDT
CIW5S16655
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
280-293Subventions
Organisme : NIEHS NIH HHS
ID : R01 ES012974
Pays : United States
Informations de copyright
Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.
Références
BMC Dev Biol. 2007 Sep 18;7:104
pubmed: 17875220
Stem Cells. 2001;19(4):287-94
pubmed: 11463948
Nucleic Acids Res. 2014 Jan;42(Database issue):D199-205
pubmed: 24214961
Environ Epigenet. 2016;2(1):
pubmed: 27390622
Biol Reprod. 2013 Aug 29;89(2):47
pubmed: 23843236
Dev Biol. 1992 Nov;154(1):160-8
pubmed: 1426623
Environ Epigenet. 2017 Apr 14;3(1):dvx003
pubmed: 29492305
Bioessays. 2014 Apr;36(4):359-71
pubmed: 24431278
Chromosome Res. 1994 Mar;2(2):147-52
pubmed: 8032673
Nucleic Acids Res. 2000 Jan 1;28(1):27-30
pubmed: 10592173
Science. 2005 Jun 3;308(5727):1466-9
pubmed: 15933200
Prog Biophys Mol Biol. 2015 Jul;118(1-2):79-85
pubmed: 25769497
Biol Reprod. 1971 Apr;4(2):195-215
pubmed: 4107186
Nat Genet. 1994 May;7(1):59-63
pubmed: 8075642
Nat Protoc. 2009;4(8):1184-91
pubmed: 19617889
Reprod Toxicol. 2008 Jan;25(1):2-6
pubmed: 17949945
PLoS One. 2014 Jul 24;9(7):e102091
pubmed: 25057798
BMC Genomics. 2014 Aug 20;15:692
pubmed: 25142051
Epigenetics Chromatin. 2017 Jul 27;10(1):38
pubmed: 28750655
Epigenetics Chromatin. 2018 Feb 27;11(1):8
pubmed: 29482626
Nucleic Acids Res. 2015 Jan;43(Database issue):D662-9
pubmed: 25352552
Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2317-21
pubmed: 2006171
Environ Epigenet. 2017 Jul;3(3):
pubmed: 29147574
Epigenetics. 2011 Jul;6(7):838-42
pubmed: 21637037
Nat Protoc. 2009;4(1):44-57
pubmed: 19131956
Cell Metab. 2017 Mar 7;25(3):544-558
pubmed: 28273477
Nat Neurosci. 2014 May;17(5):667-9
pubmed: 24728267
Nat Rev Genet. 2016 Oct;17(10):585-600
pubmed: 27573372
Bioinformatics. 2014 Aug 1;30(15):2114-20
pubmed: 24695404
PLoS One. 2013 Jul 15;8(7):e66318
pubmed: 23869203
Nat Protoc. 2013 Aug;8(8):1551-66
pubmed: 23868073
Science. 2016 Jan 22;351(6271):391-396
pubmed: 26721685
Genes Dev. 1992 May;6(5):705-14
pubmed: 1577268
Recent Prog Horm Res. 1973;29:1-41
pubmed: 4584366
Sci Rep. 2018 Mar 28;8(1):5308
pubmed: 29593303
Trends Endocrinol Metab. 2010 Apr;21(4):214-22
pubmed: 20074974
Dev Biol. 2019 Jan 15;445(2):280-293
pubmed: 30500333
Front Genet. 2018 Feb 08;9:32
pubmed: 29472946
Mol Cell. 2012 Dec 28;48(6):849-62
pubmed: 23219530
PLoS Genet. 2016 Jan 25;12(1):e1005813
pubmed: 26808625
PLoS One. 2012;7(2):e31901
pubmed: 22389676
Bioinformatics. 2010 Jan 1;26(1):139-40
pubmed: 19910308
Endocrinology. 2006 Dec;147(12):5515-23
pubmed: 16973726
BMC Med. 2013 Oct 23;11:228
pubmed: 24228800
Genome Biol Evol. 2015 Apr 26;7(5):1296-302
pubmed: 25917417
Annu Rev Genet. 2018 Nov 23;52:21-41
pubmed: 30160987
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
J Reprod Fertil Suppl. 1976 Sep;(24 suppl):115-28
pubmed: 1069848
Mol Cell Endocrinol. 2014 Dec;398(1-2):24-30
pubmed: 25224488
Hum Reprod Update. 2009 Mar-Apr;15(2):213-27
pubmed: 19136456
Environ Epigenet. 2018 Apr 26;4(2):dvy010
pubmed: 29732173
Bioinformatics. 2014 Jan 15;30(2):284-6
pubmed: 24227674
Reproduction. 2008 May;135(5):713-21
pubmed: 18304984
Dev Growth Differ. 2010 Aug;52(6):527-32
pubmed: 20646025
Biol Reprod. 2015 Feb;92(2):54
pubmed: 25568304
Biol Reprod. 2002 May;66(5):1422-9
pubmed: 11967206
Mol Cell Endocrinol. 2012 May 6;354(1-2):9-15
pubmed: 21970811
Nat Biotechnol. 2014 Sep;32(9):896-902
pubmed: 25150836