FOXP3 and CD25 double staining antibody cocktails identify regulatory T cells in different types of tumor tissues using tissue microarrays.
Antibodies
Biomarkers
/ analysis
Female
Forkhead Transcription Factors
/ analysis
Humans
Image Processing, Computer-Assisted
Immunohistochemistry
/ methods
Immunophenotyping
/ methods
Interleukin-2 Receptor alpha Subunit
/ analysis
Lymphocytes, Tumor-Infiltrating
/ immunology
Male
Staining and Labeling
/ methods
T-Lymphocytes, Regulatory
/ immunology
Tissue Array Analysis
/ methods
CD25
FOXP3
ImageJ
Immunohistochemistry
TMA
Tregs
Journal
Annals of diagnostic pathology
ISSN: 1532-8198
Titre abrégé: Ann Diagn Pathol
Pays: United States
ID NLM: 9800503
Informations de publication
Date de publication:
Feb 2019
Feb 2019
Historique:
received:
29
10
2018
accepted:
20
11
2018
pubmed:
7
12
2018
medline:
14
6
2019
entrez:
4
12
2018
Statut:
ppublish
Résumé
Regulatory T cells (Tregs) are CD4+ T cells that express CD25 and transcription factor Forkhead box P3 (FOXP3), and Tregs play a central role in regulation of tumor immunity. FOXP3 immunohistochemistry has been widely used to study Tregs in paraffin embedded tissue, and flow cytometry using fresh tissue has been used to identify FOXP3+/CD25+ double positive Tregs. In our study, we validated the FOXP3/CD25 double staining antibody cocktails for detecting Tregs in paraffin-embedded tissue. Tissue microarrays (TMA) included 115 malignant tumors, 3 ovarian mucinous borderline tumors and 15 benign tissues. Digital image analysis was performed using ImageJ software. Our results showed that FOXP3/CD25 double positive lymphocytes, a subset of FOXP3+ lymphocytes, accounted for variable percentage of the total FOXP3+ lymphocytes and they were positively correlated with FOXP3+ cell counts. Tumors from different sites had variable FOXP3+/CD25+ and FOXP3+ lymphocyte counts. Tumors from lung, head & neck and colon had more and renal cell carcinoma had minimal FOXP3+/CD25+ and FOXP3+ lymphocytes. In conclusion, FOXP3/CD25 double staining antibody cocktails can be easily applied to paraffin-embedded tissue, and FOXP3+/CD25+ Tregs count was positively correlated with FOPX3+ Tregs count but they were not interchangeable. We recommend using CD25/FOXP3 double staining for studying Tregs in tumor tissue.
Identifiants
pubmed: 30502715
pii: S1092-9134(18)30343-5
doi: 10.1016/j.anndiagpath.2018.11.005
pii:
doi:
Substances chimiques
Antibodies
0
Biomarkers
0
FOXP3 protein, human
0
Forkhead Transcription Factors
0
IL2RA protein, human
0
Interleukin-2 Receptor alpha Subunit
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
67-70Informations de copyright
Copyright © 2018 Elsevier Inc. All rights reserved.