Top-down lignomic matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry analysis of lignin oligomers extracted from date palm wood.

We report for the first time the top-down lignomic analysis of the virgin released lignin (VRL) oligomers obtained from the Saudi date palm wood (SDPW), using a matrix-assisted laser desorption/ionization time-of-flight/time-of-flight (MALDI-TOF/TOF) instrument. In addition, we are proposing new collision-induced dissociation tandem mass spectrometry (CID-MS/MS) fragmentation routes for this series of unreported VRL oligomers. We have used direct MALDI-TOF-MS analysis of the mixture of lignin oligomers without any chromatographic pre-separation. High-energy CID-MS/MS analyses were used to confirm the precursor ion structures. Six protonated lignin oligomer molecules were identified: [C To our knowledge, this is the first elucidation of the lignin structure of the Saudi seedling date palm wood that was accomplished using a top-down lignomic strategy that has not previously been published. The complex high-energy CID-MS/MS fragmentations presented herein are novel and have never been described before.

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