Examining Cellular Responses to Kinase Drug Inhibition Through Phosphoproteome Mapping of Substrates.

Chromatography, Liquid Drug Discovery / methods Enzyme Inhibitors / pharmacology Humans Mass Spectrometry Phosphopeptides / metabolism Phosphoproteins / metabolism Phosphotransferases / antagonists & inhibitors Proteomics / methods
Label-free Mass spectrometry Phosphopeptide enrichment Phosphoproteomics

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2019
Historique:
entrez: 7 12 2018
pubmed: 7 12 2018
medline: 5 6 2019
Statut: ppublish

Résumé

This chapter provides a detailed description for mass spectrometry-based phosphoproteomics analysis. We describe sample preparation, phosphopeptide enrichment, mass spectrometry acquisition, label-free data analysis and statistical analysis. This technique can be employed to characterize cell signaling networks, and is particularly useful to monitor cellular responses to kinase drug inhibitors.

Identifiants

DOI: 10.1007/978-1-4939-8891-4_8 PMID: 30519945
pubmed: 30519945
doi: 10.1007/978-1-4939-8891-4_8
doi:

Substances chimiques

Enzyme Inhibitors 0
Phosphopeptides 0
Phosphoproteins 0
Phosphotransferases EC 2.7.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

141-152

Auteurs

Daniel Bucio-Noble (D)

Department of Molecular Sciences, ARC Training Centre for Molecular Technology in the Food Industry, Macquarie University, Sydney, Australia.

Crystal Semaan (C)

Department of Molecular Sciences, ARC Training Centre for Molecular Technology in the Food Industry, Macquarie University, Sydney, Australia.

Mark P Molloy (MP)

Department of Molecular Sciences, ARC Training Centre for Molecular Technology in the Food Industry, Macquarie University, Sydney, Australia. m.molloy@sydney.edu.au.
Australian Proteome Analysis Facility, Macquarie University, Sydney, Australia. m.molloy@sydney.edu.au.
Bowel Cancer and Biomarker Research, Kolling Institute, The University of Sydney, Sydney, Australia. m.molloy@sydney.edu.au.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Examining Cellular Responses to Kinase Drug...
questionsmedicales.fr › Techniques d'investigation › Développement de médicament › Découverte de médicament › Examining Cellular Responses to Kinase Drug...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Mécanismes moléculaires de l'action pharmacologique › Antienzymes › Examining Cellular Responses to Kinase Drug...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Examining Cellular Responses to Kinase Drug...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Examining Cellular Responses to Kinase Drug...
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Phosphopeptides › Examining Cellular Responses to Kinase Drug...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Phosphoprotéines › Examining Cellular Responses to Kinase Drug...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Examining Cellular Responses to Kinase Drug...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Protéomique › Examining Cellular Responses to Kinase Drug...