A new automated device for quantifying mechanical nociceptive responses.
Automated
Nociceptive
Pain
Touch circuit
Withdrawal
Journal
Journal of neuroscience methods
ISSN: 1872-678X
Titre abrégé: J Neurosci Methods
Pays: Netherlands
ID NLM: 7905558
Informations de publication
Date de publication:
15 01 2019
15 01 2019
Historique:
received:
13
09
2018
revised:
01
12
2018
accepted:
01
12
2018
pubmed:
7
12
2018
medline:
21
3
2020
entrez:
7
12
2018
Statut:
ppublish
Résumé
Traditional methods to assess pain in rodents depend on measures of nociceptive responses, most commonly from the hind paws. While these measures can quantify nociceptive responses to allow pharmacologic testing, they typically have high inter-experimenter variability and are not time-sensitive enough to correct with neural processes that occur on millisecond scales. We have invented a pain detection device that uses changes in skin conductance to measure nocifensive withdrawal responses. This device automatically records how long it takes for a rodent to withdraw its paw from the onset of peripheral noxious stimulation. With this pain device, we can record accurate timing (on the millisecond scale) for nociceptive responses, with high accuracy and consistency. Furthermore, we demonstrate that this device can allow us to distinguish the nociceptive response to mechanical noxious stimuli of different intensities. Finally, we demonstrate that this device can be digitally integrated to correlate behavior with neural activities in real-time. This study demonstrates a new automated, temporally specific method for quantifying nociceptive responses to facilitate pain studies.
Sections du résumé
BACKGROUND
Traditional methods to assess pain in rodents depend on measures of nociceptive responses, most commonly from the hind paws. While these measures can quantify nociceptive responses to allow pharmacologic testing, they typically have high inter-experimenter variability and are not time-sensitive enough to correct with neural processes that occur on millisecond scales.
NEW METHOD
We have invented a pain detection device that uses changes in skin conductance to measure nocifensive withdrawal responses. This device automatically records how long it takes for a rodent to withdraw its paw from the onset of peripheral noxious stimulation.
RESULTS
With this pain device, we can record accurate timing (on the millisecond scale) for nociceptive responses, with high accuracy and consistency. Furthermore, we demonstrate that this device can allow us to distinguish the nociceptive response to mechanical noxious stimuli of different intensities. Finally, we demonstrate that this device can be digitally integrated to correlate behavior with neural activities in real-time.
CONCLUSIONS
This study demonstrates a new automated, temporally specific method for quantifying nociceptive responses to facilitate pain studies.
Identifiants
pubmed: 30521828
pii: S0165-0270(18)30393-5
doi: 10.1016/j.jneumeth.2018.12.001
pmc: PMC6349465
mid: NIHMS1516400
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
148-153Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM115384
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS100016
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS100065
Pays : United States
Informations de copyright
Published by Elsevier B.V.
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