Seroepidemiological and molecular investigations of infections with Crimean-Congo haemorrhagic fever virus in Kazakhstan.
Adolescent
Adult
Animals
Antibodies, Viral
/ blood
Cattle
Enzyme-Linked Immunosorbent Assay
Female
Fever of Unknown Origin
/ etiology
Hemorrhagic Fever Virus, Crimean-Congo
/ genetics
Hemorrhagic Fever, Crimean
/ complications
Horses
Humans
Kazakhstan
/ epidemiology
Male
Middle Aged
RNA, Viral
/ blood
Seroepidemiologic Studies
Young Adult
Crimean–Congo haemorrhagic fever virus
Fever of unknown origin
Kazakhstan
Reassortment
Journal
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
ISSN: 1878-3511
Titre abrégé: Int J Infect Dis
Pays: Canada
ID NLM: 9610933
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
19
07
2018
revised:
15
10
2018
accepted:
19
10
2018
pubmed:
12
12
2018
medline:
6
2
2019
entrez:
8
12
2018
Statut:
ppublish
Résumé
The aim of this study was to detect the seroprevalence of Crimean-Congo haemorrhagic fever virus (CCHFV) in patients with fever of unknown origin (FUO) in endemic (Kyzylorda) and non-endemic (Almaty) oblasts of Kazakhstan. Paired serum samples from 802 patients with FUO were collected. Serum samples were investigated by ELISA to detect IgG and IgM antibodies against CCHFV. Sera with suspected acute infection were further investigated by RT-PCR to detect the viral RNA. IgG antibodies were detected in 12.7% of the sera from both oblasts. Acute infection was shown by IgM ELISA in four patients from Kyzylorda, with only one developing severe CCHF. Viral RNA was found by RT-PCR in the other three patients' sera. Phylogenetic analysis of partial L and S segments revealed CCHFV genotype Asia 2 and a possible reassortment between the genotypes Asia 1/Asia 2. Animal husbandry, such as working with cattle and horses, was significantly associated with CCHFV seropositivity. The antibodies and viral RNA detected in sera indicate that mild or even asymptomatic CCHFV infections are presented in Kazakhstan. This study describes the circulation of CCHFV in the so far non-endemic Almaty oblast for the first time. In conclusion, physicians treating patients with FUO in Kazakhstan should be aware of mild CCHF.
Identifiants
pubmed: 30522982
pii: S1201-9712(18)34561-2
doi: 10.1016/j.ijid.2018.10.015
pii:
doi:
Substances chimiques
Antibodies, Viral
0
RNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
121-127Informations de copyright
Copyright © 2018 The Author(s). Published by Elsevier Ltd.. All rights reserved.