Differential expression of genes in greenbug (Schizaphis graminum Rondani) treated by imidacloprid and RNA interference.
Greenbug
RNA interference (RNAi)
RNA-seq
differential expression
digital gene expression (DGE)
Journal
Pest management science
ISSN: 1526-4998
Titre abrégé: Pest Manag Sci
Pays: England
ID NLM: 100898744
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
received:
29
04
2018
revised:
21
11
2018
accepted:
01
12
2018
pubmed:
14
12
2018
medline:
27
6
2019
entrez:
8
12
2018
Statut:
ppublish
Résumé
Insecticides act as toxins, inhibitors of digestion and deterrents, and affect the expression of many genes in insects. To assess key genes associated with the detoxification or regulation of imidacloprid in greenbug, Schizaphis graminum (Rondani), the transcriptome and digital gene expression (DGE) profile were analyzed using Illumina sequencing. In total, 48 763 494 clean reads were obtained by sequencing. Expression profile analysis showed that 2782 unigenes were differently expressed between the imidacloprid treatment and control groups. After exposure to imidacloprid, the expression levels of 1846 unigenes were upregulated and 936 were downregulated in comparison with controls. Expression patterns of the top 20 highly expressed genes show that they could be involved in the detoxification of imidacloprid. Silencing of multidrug resistance-associated gene (MRA), GATA-binding gene (GAT) and takeout-like precursor gene (TLP) resulted in increasing susceptibility to imidacloprid. The differentially expressed genes in S. graminum have potential regulatory or detoxification roles in response to imidacloprid. These results should be useful in understanding the molecular mechanisms of greenbug adaption to imidacloprid. © 2018 Society of Chemical Industry.
Sections du résumé
BACKGROUND
BACKGROUND
Insecticides act as toxins, inhibitors of digestion and deterrents, and affect the expression of many genes in insects. To assess key genes associated with the detoxification or regulation of imidacloprid in greenbug, Schizaphis graminum (Rondani), the transcriptome and digital gene expression (DGE) profile were analyzed using Illumina sequencing.
RESULTS
RESULTS
In total, 48 763 494 clean reads were obtained by sequencing. Expression profile analysis showed that 2782 unigenes were differently expressed between the imidacloprid treatment and control groups. After exposure to imidacloprid, the expression levels of 1846 unigenes were upregulated and 936 were downregulated in comparison with controls. Expression patterns of the top 20 highly expressed genes show that they could be involved in the detoxification of imidacloprid. Silencing of multidrug resistance-associated gene (MRA), GATA-binding gene (GAT) and takeout-like precursor gene (TLP) resulted in increasing susceptibility to imidacloprid.
CONCLUSIONS
CONCLUSIONS
The differentially expressed genes in S. graminum have potential regulatory or detoxification roles in response to imidacloprid. These results should be useful in understanding the molecular mechanisms of greenbug adaption to imidacloprid. © 2018 Society of Chemical Industry.
Substances chimiques
Insect Proteins
0
Insecticides
0
Neonicotinoids
0
Nitro Compounds
0
imidacloprid
3BN7M937V8
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1726-1733Subventions
Organisme : The National Key Research and Development Program of China
ID : 2017YFD0201700
Organisme : The Key Science and Technology Program of Henan (Agriculture)
ID : 182102110053
Organisme : The Project of High-level Talent Introduction of Henan Institute of Science and Technology, China
ID : 208010616003
Organisme : The Scientific and Technological Innovation of Henan Institute of Science and Technology, China
ID : 208010916005
Informations de copyright
© 2018 Society of Chemical Industry.